Detailed information of TA system TA145846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 337479..337977
• Replicon: chromosome
• Accession: NZ_CP047300
• Organism: Vibrio cholerae O1 biovar El Tor strain P27459

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: GTF71_RS14990
• Protein ID: WP_000589156.1
• Coordinates: 337479..337745 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: GTF71_RS14995
• Protein ID: WP_000643598.1
• Coordinates: 337732..337977 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF71_RS14935	332900..333106	+	207	Protein_297	DUF3709 domain-containing protein	-
GTF71_RS14940	333306..333407	+	102	WP_001921603.1	hypothetical protein	-
GTF71_RS14945	333397..333783	+	387	WP_000703163.1	VOC family protein	-
GTF71_RS14950	333978..334229	+	252	WP_001890111.1	TIGR03643 family protein	-
GTF71_RS14955	334202..334351	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
GTF71_RS14960	334443..334685	+	243	WP_000107461.1	hypothetical protein	-
GTF71_RS14965	334937..335215	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
GTF71_RS14970	335590..335733	+	144	Protein_304	DUF645 family protein	-
GTF71_RS14975	335787..335825	+	39	WP_082798268.1	hypothetical protein	-
GTF71_RS14980	336037..336504	+	468	WP_001289288.1	OsmC family protein	-
GTF71_RS14985	336632..337294	+	663	WP_000960654.1	hypothetical protein	-
GTF71_RS14990	337479..337745	+	267	WP_000589156.1	BrnT family toxin	Toxin
GTF71_RS14995	337732..337977	+	246	WP_000643598.1	hypothetical protein	Antitoxin
GTF71_RS15000	338073..338867	+	795	WP_001911581.1	hypothetical protein	-
GTF71_RS15005	338970..339098	+	129	WP_071908338.1	general secretion pathway protein GspI	-
GTF71_RS15010	339159..339341	+	183	WP_000923182.1	DUF645 family protein	-
GTF71_RS15015	339557..340561	+	1005	WP_000964920.1	LD-carboxypeptidase	-
GTF71_RS15020	340714..341073	+	360	WP_001071541.1	VOC family protein	-
GTF71_RS15025	341346..341579	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
GTF71_RS15030	341847..342353	+	507	WP_000393074.1	hypothetical protein	-
GTF71_RS15035	342510..342896	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	306926..439292	132366
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	312006..438916	126910

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T145846 WP_000589156.1 NZ_CP047300:337479-337745 [Vibrio cholerae O1 biovar El Tor]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T145846 NZ_CP062702:c3305701-3305327 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT145846 WP_000643598.1 NZ_CP047300:337732-337977 [Vibrio cholerae O1 biovar El Tor]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT145846 NZ_CP062702:c3306122-3305748 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2