Detailed information of TA system TA058316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 578988..580081
• Replicon: chromosome
• Accession: NZ_CP013310
• Organism: Vibrio cholerae strain E1162

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: ASZ87_RS17660
• Protein ID: WP_000351248.1
• Coordinates: 579680..580081 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: ASZ87_RS17655
• Protein ID: WP_001047169.1
• Coordinates: 578988..579680 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ87_RS17585	574271..574648	+	378	WP_000411109.1	hypothetical protein	-
ASZ87_RS21130	574705..574819	+	115	Protein_601	acetyltransferase	-
ASZ87_RS17590	574768..575049	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
ASZ87_RS17605	575215..575328	+	114	WP_001889158.1	hypothetical protein	-
ASZ87_RS17610	575277..575504	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
ASZ87_RS17620	575845..576177	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ87_RS17625	576164..576478	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
ASZ87_RS17630	576615..577049	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
ASZ87_RS20915	577217..577372	+	156	WP_000751734.1	hypothetical protein	-
ASZ87_RS17645	577497..578477	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
ASZ87_RS17650	578545..578851	+	307	Protein_610	CatB-related O-acetyltransferase	-
ASZ87_RS17655	578988..579680	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
ASZ87_RS17660	579680..580081	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ASZ87_RS20820	580051..580194	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
ASZ87_RS17670	580269..580682	+	414	WP_000049417.1	VOC family protein	-
ASZ87_RS17675	580896..581147	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ87_RS17680	581137..581424	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ87_RS17685	581552..582007	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ87_RS17695	582329..582481	+	153	WP_001884520.1	DUF645 family protein	-
ASZ87_RS17700	582683..583180	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
ASZ87_RS17705	583177..583449	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
ASZ87_RS17710	583680..584348	+	669	WP_000043871.1	hypothetical protein	-
ASZ87_RS17715	584500..584787	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	435534..596659	161125
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	440614..596283	155669
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	577497..578477	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T58316 WP_000351248.1 NZ_CP013310:579680-580081 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T58316 NZ_CP013310:579680-580081 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT58316 WP_001047169.1 NZ_CP013310:578988-579680 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT58316 NZ_CP013310:578988-579680 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34