Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 253938..254553 | Replicon | chromosome |
| Accession | NZ_AP024554 | ||
| Organism | Vibrio cholerae strain IDH-03329 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | Q9KMG5 |
| Locus tag | K6J80_RS15145 | Protein ID | WP_001162670.1 |
| Coordinates | 254266..254553 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | K6J80_RS15140 | Protein ID | WP_001232701.1 |
| Coordinates | 253938..254255 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K6J80_RS15105 | 249347..249808 | - | 462 | WP_001911752.1 | lipocalin family protein | - |
| K6J80_RS15110 | 249999..250475 | - | 477 | WP_001047180.1 | GNAT family N-acetyltransferase | - |
| K6J80_RS18985 | 250503..250613 | - | 111 | WP_071917832.1 | DUF3265 domain-containing protein | - |
| K6J80_RS15115 | 250610..251539 | - | 930 | WP_000335802.1 | hypothetical protein | - |
| K6J80_RS15120 | 251659..252105 | - | 447 | WP_000128977.1 | hypothetical protein | - |
| K6J80_RS15125 | 252258..253262 | - | 1005 | WP_000964925.1 | LD-carboxypeptidase | - |
| K6J80_RS18990 | 253450..253566 | - | 117 | WP_001889170.1 | hypothetical protein | - |
| K6J80_RS15135 | 253529..253654 | - | 126 | WP_001944767.1 | DUF645 family protein | - |
| K6J80_RS18995 | 253682..253792 | - | 111 | Protein_262 | acetyltransferase | - |
| K6J80_RS19000 | 253831..253941 | - | 111 | WP_134814058.1 | DUF3265 domain-containing protein | - |
| K6J80_RS15140 | 253938..254255 | - | 318 | WP_001232701.1 | HigA family addiction module antitoxin | Antitoxin |
| K6J80_RS15145 | 254266..254553 | - | 288 | WP_001162670.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| K6J80_RS19005 | 254594..254707 | - | 114 | WP_001894955.1 | hypothetical protein | - |
| K6J80_RS15150 | 254820..255000 | - | 181 | Protein_267 | DUF645 family protein | - |
| K6J80_RS15155 | 255425..255658 | - | 234 | WP_032482776.1 | DUF3709 domain-containing protein | - |
| K6J80_RS19010 | 255679..255872 | - | 194 | Protein_269 | hypothetical protein | - |
| K6J80_RS15160 | 256061..256279 | - | 219 | WP_001917086.1 | DUF3709 domain-containing protein | - |
| K6J80_RS19015 | 256291..256500 | - | 210 | WP_001900246.1 | DUF3709 domain-containing protein | - |
| K6J80_RS15165 | 256589..257242 | - | 654 | WP_000226874.1 | hypothetical protein | - |
| K6J80_RS15170 | 257299..257808 | - | 510 | WP_000405987.1 | GNAT family N-acetyltransferase | - |
| K6J80_RS15175 | 258050..258292 | + | 243 | WP_000557292.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| K6J80_RS15180 | 258311..258628 | + | 318 | WP_001180243.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| K6J80_RS19020 | 258822..258974 | - | 153 | Protein_276 | DUF645 family protein | - |
| K6J80_RS19025 | 259035..259210 | - | 176 | Protein_277 | acetyltransferase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | catB9 | - | 213119..319686 | 106567 | |
| inside | Integron | - | - | 213119..312856 | 99737 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11349.95 Da Isoelectric Point: 6.7187
>T38677 WP_001162670.1 NZ_AP024554:c254553-254266 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY
Download Length: 288 bp
>T38677 NZ_AP024554:c254553-254266 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11705.63 Da Isoelectric Point: 10.4711
>AT38677 WP_001232701.1 NZ_AP024554:c254255-253938 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART
Download Length: 318 bp
>AT38677 NZ_AP024554:c254255-253938 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|