Detailed information of TA system TA058407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 485540..486495
• Replicon: chromosome
• Accession: NZ_CP013318
• Organism: Vibrio cholerae strain NCTC5395

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: ASZ85_RS17125
• Protein ID: WP_000118351.1
• Coordinates: 485962..486495 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ASZ85_RS17120
• Protein ID: WP_001882332.1
• Coordinates: 485540..485965 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ85_RS17070	481070..481321	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ85_RS17075	481311..481598	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ85_RS17080	481726..482181	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ85_RS17090	482503..482655	+	153	WP_001884520.1	DUF645 family protein	-
ASZ85_RS17095	482857..483354	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
ASZ85_RS17100	483351..483623	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
ASZ85_RS17105	483854..484522	+	669	WP_000043871.1	hypothetical protein	-
ASZ85_RS17110	484674..484961	+	288	WP_000426470.1	hypothetical protein	-
ASZ85_RS17115	485102..485473	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ85_RS17120	485540..485965	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
ASZ85_RS17125	485962..486495	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
ASZ85_RS17130	486697..486954	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ85_RS17135	486942..487244	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ85_RS17140	487478..488395	+	918	WP_000186333.1	alpha/beta hydrolase	-
ASZ85_RS17150	488552..488941	+	390	WP_001081302.1	hypothetical protein	-
ASZ85_RS17155	489077..489286	+	210	Protein_549	GNAT family N-acetyltransferase	-
ASZ85_RS17160	489405..489842	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
ASZ85_RS17165	489906..490124	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
ASZ85_RS17175	490299..491171	+	873	WP_000254716.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	338285..496833	158548
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.009	inside	Integron	-	-	343365..496457	153092
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	489405..489842	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T58407 WP_000118351.1 NZ_CP013318:485962-486495 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T58407 NZ_CP013318:485962-486495 [Vibrio cholerae]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT58407 WP_001882332.1 NZ_CP013318:485540-485965 [Vibrio cholerae]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT58407 NZ_CP013318:485540-485965 [Vibrio cholerae]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94