Detailed information of TA system TA145826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 334809..335307
• Replicon: chromosome
• Accession: NZ_CP047298
• Organism: Vibrio cholerae O1 biovar El Tor strain C6709

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: GTF73_RS15310
• Protein ID: WP_000589156.1
• Coordinates: 334809..335075 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: GTF73_RS15315
• Protein ID: WP_000643598.1
• Coordinates: 335062..335307 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF73_RS15255	330229..330435	+	207	Protein_294	DUF3709 domain-containing protein	-
GTF73_RS15260	330635..330736	+	102	WP_001921603.1	hypothetical protein	-
GTF73_RS15265	330726..331112	+	387	WP_000703163.1	VOC family protein	-
GTF73_RS15270	331307..331558	+	252	WP_001890111.1	TIGR03643 family protein	-
GTF73_RS15275	331531..331680	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
GTF73_RS15280	331772..332014	+	243	WP_000107461.1	hypothetical protein	-
GTF73_RS15285	332266..332544	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
GTF73_RS15290	332919..333062	+	144	Protein_301	DUF645 family protein	-
GTF73_RS15295	333116..333154	+	39	WP_082798268.1	hypothetical protein	-
GTF73_RS15300	333366..333833	+	468	WP_001289288.1	OsmC family protein	-
GTF73_RS15305	333961..334624	+	664	Protein_304	hypothetical protein	-
GTF73_RS15310	334809..335075	+	267	WP_000589156.1	BrnT family toxin	Toxin
GTF73_RS15315	335062..335307	+	246	WP_000643598.1	hypothetical protein	Antitoxin
GTF73_RS15320	335403..336197	+	795	WP_001911581.1	hypothetical protein	-
GTF73_RS15325	336300..336428	+	129	WP_071908338.1	general secretion pathway protein GspI	-
GTF73_RS15330	336489..336671	+	183	WP_000923182.1	DUF645 family protein	-
GTF73_RS15335	336887..337891	+	1005	WP_000964920.1	LD-carboxypeptidase	-
GTF73_RS15340	338044..338403	+	360	WP_001071541.1	VOC family protein	-
GTF73_RS15345	338676..338909	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
GTF73_RS15350	339177..339683	+	507	WP_000393074.1	hypothetical protein	-
GTF73_RS15355	339840..340226	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	306901..436162	129261
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	311981..435786	123805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T145826 WP_000589156.1 NZ_CP047298:334809-335075 [Vibrio cholerae O1 biovar El Tor]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T145826 NZ_CP062700:c4936915-4936565 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT145826 WP_000643598.1 NZ_CP047298:335062-335307 [Vibrio cholerae O1 biovar El Tor]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT145826 NZ_CP062700:c4937160-4936909 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2