58394

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 375325..376091 Replicon chromosome
Accession NZ_CP013318
Organism Vibrio cholerae strain NCTC5395

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag ASZ85_RS15990 Protein ID WP_000982260.1
Coordinates 375325..375822 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag ASZ85_RS15995 Protein ID WP_000246253.1
Coordinates 375819..376091 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ85_RS15940 370767..371471 + 705 WP_000087610.1 HNH endonuclease -
ASZ85_RS15945 371629..371967 + 339 WP_000713853.1 hypothetical protein -
ASZ85_RS15950 372121..372438 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
ASZ85_RS15955 372457..372699 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ASZ85_RS15960 372943..373329 + 387 WP_001015867.1 NUDIX hydrolase -
ASZ85_RS15965 373386..373499 + 114 WP_001900214.1 hypothetical protein -
ASZ85_RS15970 373448..373729 + 282 WP_001894429.1 DUF1289 domain-containing protein -
ASZ85_RS15980 373987..374523 + 537 WP_000469482.1 GNAT family N-acetyltransferase -
ASZ85_RS15985 374660..375175 + 516 WP_001201509.1 lipocalin family protein -
ASZ85_RS15990 375325..375822 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
ASZ85_RS15995 375819..376091 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
ASZ85_RS20980 376484..376609 + 126 WP_001900195.1 DUF645 family protein -
ASZ85_RS20985 376625..376663 + 39 WP_106019118.1 hypothetical protein -
ASZ85_RS16005 376859..377305 + 447 WP_000006157.1 hypothetical protein -
ASZ85_RS16010 377453..377722 - 270 WP_000277238.1 type II toxin-antitoxin system YafQ family toxin -
ASZ85_RS16015 377715..377993 - 279 WP_001258569.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
ASZ85_RS16020 378243..378500 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ASZ85_RS16025 378488..378790 + 303 WP_000229318.1 type II toxin-antitoxin system RelE/ParE family toxin -
ASZ85_RS16030 379079..379303 + 225 WP_001894423.1 DUF3709 domain-containing protein -
ASZ85_RS16035 379747..379866 + 120 WP_071919600.1 DUF645 family protein -
ASZ85_RS21000 379870..379923 + 54 Protein_360 DUF645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 338285..496833 158548
inside Integron - - 343365..496457 153092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T58394 WP_000982260.1 NZ_CP013318:c375822-375325 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T58394 NZ_CP013318:c375822-375325 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT58394 WP_000246253.1 NZ_CP013318:c376091-375819 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT58394 NZ_CP013318:c376091-375819 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References