Detailed information of TA system TA048157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 395916..396682
• Replicon: chromosome
• Accession: NZ_CP009041
• Organism: Vibrio cholerae O1 biovar El Tor strain FJ147

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: IR04_RS02005
• Protein ID: WP_000982260.1
• Coordinates: 395916..396413 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2J6
• Locus tag: IR04_RS02010
• Protein ID: WP_000246253.1
• Coordinates: 396410..396682 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IR04_RS01965	391778..392050	+	273	Protein_404	CatB-related O-acetyltransferase	-
IR04_RS01970	392221..392913	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
IR04_RS01975	392913..393314	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
IR04_RS20025	393284..393427	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
IR04_RS01980	393502..393915	+	414	WP_000049417.1	VOC family protein	-
IR04_RS01985	394129..394380	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IR04_RS01990	394370..394657	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IR04_RS01995	394785..395240	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
IR04_RS02000	395562..395714	+	153	WP_001884520.1	DUF645 family protein	-
IR04_RS02005	395916..396413	-	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
IR04_RS02010	396410..396682	-	273	WP_000246253.1	DUF1778 domain-containing protein	Antitoxin
IR04_RS02015	396913..397581	+	669	WP_000043871.1	hypothetical protein	-
IR04_RS02020	397733..398020	+	288	WP_000426470.1	hypothetical protein	-
IR04_RS02025	398161..398532	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
IR04_RS02030	398599..399024	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
IR04_RS02035	399021..399554	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
IR04_RS02040	399756..400013	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IR04_RS02045	400001..400303	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IR04_RS02050	400537..401454	+	918	WP_000186333.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304700..409892	105192
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	309780..409516	99736
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	390730..391710	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T48157 WP_000982260.1 NZ_CP009041:c396413-395916 [Vibrio cholerae O1 biovar El Tor]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T48157 NZ_CP009041:c396413-395916 [Vibrio cholerae O1 biovar El Tor]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9683.22 Da
• Isoelectric Point: 6.2988

>AT48157 WP_000246253.1 NZ_CP009041:c396682-396410 [Vibrio cholerae O1 biovar El Tor]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT48157 NZ_CP009041:c396682-396410 [Vibrio cholerae O1 biovar El Tor]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1

Antitoxin

• AlphaFold DB: A0A0F2IC79