46749

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-Phd
Location 47677..48255 Replicon chromosome
Accession NZ_CP007635
Organism Vibrio cholerae strain 2012EL-2176

Toxin (Protein)


Gene name parE Uniprot ID O68848
Locus tag EN18_RS14370 Protein ID WP_001180243.1
Coordinates 47677..47994 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID Q7DCR7
Locus tag EN18_RS14375 Protein ID WP_000557292.1
Coordinates 48013..48255 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EN18_RS14335 43201..43383 + 183 WP_000923340.1 DUF645 family protein -
EN18_RS14345 43737..43919 + 183 WP_000923153.1 DUF645 family protein -
EN18_RS14350 44114..44791 + 678 WP_000254617.1 HNH endonuclease -
EN18_RS14355 44953..46161 + 1209 WP_000272282.1 deoxyguanosinetriphosphate triphosphohydrolase family protein -
EN18_RS14360 46323..47027 + 705 WP_000087610.1 HNH endonuclease -
EN18_RS14365 47185..47523 + 339 WP_000713853.1 hypothetical protein -
EN18_RS14370 47677..47994 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EN18_RS14375 48013..48255 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EN18_RS14380 48499..48885 + 387 WP_001015867.1 NUDIX hydrolase -
EN18_RS20495 48942..49055 + 114 WP_001900214.1 hypothetical protein -
EN18_RS14385 49004..49285 + 282 WP_001894429.1 DUF1289 domain-containing protein -
EN18_RS14395 49543..50079 + 537 WP_000469482.1 GNAT family N-acetyltransferase -
EN18_RS14400 50216..50731 + 516 WP_001201509.1 lipocalin family protein -
EN18_RS14405 50881..51378 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
EN18_RS14410 51375..51647 - 273 WP_000246253.1 DUF1778 domain-containing protein -
EN18_RS21085 52040..52165 + 126 WP_001900195.1 DUF645 family protein -
EN18_RS21090 52181..52219 + 39 WP_106019118.1 hypothetical protein -
EN18_RS14425 52415..52861 + 447 WP_000006157.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 31037..137542 106505
inside Integron - - 36117..137166 101049
- flank IS/Tn - - 52924..54135 1211


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-93)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-71)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12158.97 Da        Isoelectric Point: 9.7495

>T46749 WP_001180243.1 NZ_CP007635:c47994-47677 [Vibrio cholerae]
MQNKQYKLSQLAQEHLLKIKHYTIENFAEAQWQKYKSTLLSGFQTLADNPGLGKSCEDIYQNGFYFPVGKHMAYYTKEAN
FILIVAVLGQSQLPQKHLKQSRFVS

Download         Length: 318 bp

>T46749 NZ_CP007635:c47994-47677 [Vibrio cholerae]
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTTGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTGGGGAAACACATGGCTTATTACACCAAAGAAGCAAAC
TTCATTCTCATTGTTGCGGTATTAGGGCAATCACAACTGCCCCAAAAGCATCTCAAACAGTCACGCTTTGTTTCTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8961.25 Da        Isoelectric Point: 5.6630

>AT46749 WP_000557292.1 NZ_CP007635:c48255-48013 [Vibrio cholerae]
MHTLTANDAKRNFGELLLSAQREPVIISKNSKNTVVVMSIKDFEELEAMKLDYLKHCFESAQKDLDSGKTVDGATFLNTL

Download         Length: 243 bp

>AT46749 NZ_CP007635:c48255-48013 [Vibrio cholerae]
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAACGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGACTTTGAAGAACTTGAAGCAATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCACTTTTCTAAACACCCTT
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K9UJR8

Antitoxin

Source ID Structure
AlphaFold DB Q7DCR7

References