Detailed information of TA system TA092509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 366990..367605
• Replicon: chromosome
• Accession: NZ_CP025937
• Organism: Vibrio cholerae strain A1552

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: C1H56_RS16200
• Protein ID: WP_001162670.1
• Coordinates: 366990..367277 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C1H56_RS16205
• Protein ID: WP_001232701.1
• Coordinates: 367288..367605 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1H56_RS19960	362626..362721	+	96	WP_001907607.1	DUF645 family protein	-
C1H56_RS16160	362915..363232	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C1H56_RS16165	363251..363493	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
C1H56_RS16170	363735..364244	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
C1H56_RS16175	364301..364954	+	654	WP_000226874.1	hypothetical protein	-
C1H56_RS16180	365264..365482	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
C1H56_RS16185	365885..366118	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
C1H56_RS16190	366543..366723	+	181	Protein_360	DUF645 family protein	-
C1H56_RS16200	366990..367277	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1H56_RS16205	367288..367605	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
C1H56_RS19965	367602..367712	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
C1H56_RS16220	367889..368014	+	126	WP_001944767.1	DUF645 family protein	-
C1H56_RS19855	368030..368068	+	39	WP_106019119.1	hypothetical protein	-
C1H56_RS19970	368145..368246	+	102	Protein_366	exlusion protein FxsA	-
C1H56_RS16230	368292..369032	+	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
C1H56_RS16235	369237..370067	+	831	WP_000056616.1	abortive infection family protein	-
C1H56_RS16240	370124..370606	+	483	WP_001895003.1	hypothetical protein	-
C1H56_RS16250	371053..371928	+	876	WP_000259044.1	hypothetical protein	-
C1H56_RS16255	372058..372513	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304067..433328	129261
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	catB9	-	309147..432952	123805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T92509 WP_001162670.1 NZ_CP025937:366990-367277 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T92509 NZ_CP025937:366990-367277 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT92509 WP_001232701.1 NZ_CP025937:367288-367605 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT92509 NZ_CP025937:367288-367605 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36