Detailed information of TA system TA087019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 332133..332631
• Replicon: chromosome
• Accession: NZ_CP024163
• Organism: Vibrio cholerae strain E7946

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: CSW01_RS15755
• Protein ID: WP_000589156.1
• Coordinates: 332133..332399 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: CSW01_RS15760
• Protein ID: WP_000643598.1
• Coordinates: 332386..332631 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSW01_RS19805	327554..327760	+	207	Protein_289	DUF3709 domain-containing protein	-
CSW01_RS15690	327960..328061	+	102	WP_001921603.1	hypothetical protein	-
CSW01_RS15695	328051..328437	+	387	WP_000703163.1	VOC family protein	-
CSW01_RS15700	328632..328883	+	252	WP_001890111.1	TIGR03643 family protein	-
CSW01_RS15705	328856..329005	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
CSW01_RS15710	329097..329339	+	243	WP_000107461.1	hypothetical protein	-
CSW01_RS15715	329591..329869	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
CSW01_RS19945	330244..330387	+	144	Protein_296	DUF645 family protein	-
CSW01_RS19950	330441..330479	+	39	WP_082798268.1	hypothetical protein	-
CSW01_RS15740	330691..331158	+	468	WP_001289288.1	OsmC family protein	-
CSW01_RS15745	331286..331948	+	663	WP_000960654.1	hypothetical protein	-
CSW01_RS15755	332133..332399	+	267	WP_000589156.1	BrnT family toxin	Toxin
CSW01_RS15760	332386..332631	+	246	WP_000643598.1	hypothetical protein	Antitoxin
CSW01_RS15765	332727..333521	+	795	WP_001911581.1	hypothetical protein	-
CSW01_RS19955	333624..333752	+	129	WP_071908338.1	general secretion pathway protein GspI	-
CSW01_RS15780	333813..333995	+	183	WP_000923182.1	DUF645 family protein	-
CSW01_RS15785	334211..335215	+	1005	WP_000964920.1	LD-carboxypeptidase	-
CSW01_RS15795	335368..335727	+	360	WP_001071541.1	VOC family protein	-
CSW01_RS15800	336000..336233	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
CSW01_RS15805	336501..337007	+	507	WP_000393074.1	hypothetical protein	-
CSW01_RS15810	337164..337550	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304226..433944	129718
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	309306..433568	124262

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T87019 WP_000589156.1 NZ_CP024163:332133-332399 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T87019 NZ_CP024163:332133-332399 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT87019 WP_000643598.1 NZ_CP024163:332386-332631 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT87019 NZ_CP024163:332386-332631 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2