Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 447788..448316 Replicon chromosome
Accession NZ_CP013306
Organism Vibrio cholerae strain CRC1106

Toxin (Protein)


Gene name relE Uniprot ID A0A0X1KZS6
Locus tag ASZ81_RS16980 Protein ID WP_000221354.1
Coordinates 448029..448316 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0X1KZS8
Locus tag ASZ81_RS16975 Protein ID WP_001250179.1
Coordinates 447788..448039 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ81_RS16925 443056..443370 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
ASZ81_RS16930 443507..443941 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
ASZ81_RS20760 444109..444264 + 156 WP_000751734.1 hypothetical protein -
ASZ81_RS16945 444389..445369 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
ASZ81_RS16950 445437..445743 + 307 Protein_494 CatB-related O-acetyltransferase -
ASZ81_RS16955 445880..446572 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
ASZ81_RS16960 446572..446973 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
ASZ81_RS20675 446943..447086 + 144 WP_071908341.1 DUF3265 domain-containing protein -
ASZ81_RS16970 447161..447574 + 414 WP_000049417.1 VOC family protein -
ASZ81_RS16975 447788..448039 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ASZ81_RS16980 448029..448316 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ASZ81_RS16985 448444..448899 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
ASZ81_RS16995 449221..449373 + 153 WP_001884520.1 DUF645 family protein -
ASZ81_RS17000 449575..450072 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
ASZ81_RS17005 450069..450341 - 273 WP_000246253.1 DUF1778 domain-containing protein -
ASZ81_RS17010 450572..451240 + 669 WP_000043871.1 hypothetical protein -
ASZ81_RS17015 451392..451679 + 288 WP_000426470.1 hypothetical protein -
ASZ81_RS17020 451820..452191 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
ASZ81_RS17025 452258..452683 + 426 WP_001882332.1 DUF1778 domain-containing protein -
ASZ81_RS17030 452680..453213 + 534 WP_000118351.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 304741..463550 158809
inside Integron - - 309821..463174 153353
flank IS/Tn - - 444389..445369 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(11-71)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10991.96 Da        Isoelectric Point: 10.4934

>T58274 WP_000221354.1 NZ_CP013306:448029-448316 [Vibrio cholerae]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Download         Length: 288 bp

>T58274 NZ_CP013306:448029-448316 [Vibrio cholerae]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9136.29 Da        Isoelectric Point: 4.0197

>AT58274 WP_001250179.1 NZ_CP013306:447788-448039 [Vibrio cholerae]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL

Download         Length: 252 bp

>AT58274 NZ_CP013306:447788-448039 [Vibrio cholerae]
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X1KZS6

Antitoxin

Source ID Structure
AlphaFold DB A0A0X1KZS8

References