145873

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-MqsA
Location 390322..390955 Replicon chromosome
Accession NZ_CP047302
Organism Vibrio cholerae O1 biovar El Tor strain DRC-193A

Toxin (Protein)


Gene name higB Uniprot ID Q9KMA6
Locus tag GTF75_RS15930 Protein ID WP_000843587.1
Coordinates 390322..390654 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GTF75_RS15935 Protein ID WP_000071008.1
Coordinates 390641..390955 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTF75_RS15865 385763..385966 + 204 WP_001911745.1 hypothetical protein -
GTF75_RS15870 386247..386420 + 174 WP_001882305.1 DUF3709 domain-containing protein -
GTF75_RS15875 386780..387049 + 270 WP_001198131.1 hypothetical protein -
GTF75_RS15880 387360..387446 + 87 WP_134820423.1 DUF645 family protein -
GTF75_RS15885 387446..387538 + 93 Protein_396 DUF645 family protein -
GTF75_RS15890 387747..388133 + 387 WP_000703163.1 VOC family protein -
GTF75_RS15895 388335..388577 + 243 WP_000107461.1 hypothetical protein -
GTF75_RS15900 388748..389125 + 378 WP_000411109.1 hypothetical protein -
GTF75_RS15905 389305..389526 + 222 WP_032468461.1 DUF1289 domain-containing protein -
GTF75_RS15910 389502..389660 - 159 WP_001894455.1 DUF1196 family protein -
GTF75_RS15915 389692..389805 + 114 WP_001889158.1 hypothetical protein -
GTF75_RS15920 389754..389981 + 228 WP_001894457.1 DUF1289 domain-containing protein -
GTF75_RS15925 390011..390169 - 159 WP_001882309.1 DUF1196 family protein -
GTF75_RS15930 390322..390654 + 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GTF75_RS15935 390641..390955 + 315 WP_000071008.1 DNA-binding transcriptional regulator Antitoxin
GTF75_RS15940 391092..391526 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
GTF75_RS15945 391694..391849 + 156 WP_000751734.1 hypothetical protein -
GTF75_RS15950 391974..392954 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
GTF75_RS15955 393022..393294 + 273 Protein_410 CatB-related O-acetyltransferase -
GTF75_RS15960 393465..394157 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
GTF75_RS15965 394157..394558 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
GTF75_RS15970 394528..394671 + 144 WP_071908341.1 DUF3265 domain-containing protein -
GTF75_RS15975 394746..395159 + 414 WP_000049417.1 VOC family protein -
GTF75_RS15980 395373..395624 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTF75_RS15985 395614..395901 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 305935..411136 105201
inside Integron - - 311015..410760 99745
flank IS/Tn - - 391974..392954 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(4-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(43-92)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 13005.95 Da        Isoelectric Point: 9.9244

>T145873 WP_000843587.1 NZ_CP047302:390322-390654 [Vibrio cholerae O1 biovar El Tor]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Download         Length: 333 bp

>T145873 NZ_CP062705:c2856672-2856496 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11696.20 Da        Isoelectric Point: 6.7600

>AT145873 WP_000071008.1 NZ_CP047302:390641-390955 [Vibrio cholerae O1 biovar El Tor]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Download         Length: 315 bp

>AT145873 NZ_CP062705:c2856450-2856034 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5JA9
PDB 8A0X
PDB 5MJE
PDB 5JA8
PDB 5JAA

Antitoxin

Source ID Structure

References