Detailed information of TA system TA026152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 414768..415401
• Replicon: chromosome II
• Accession: NC_016945
• Organism: Vibrio cholerae IEC224

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: O3Y_RS16125
• Protein ID: WP_000843587.1
• Coordinates: 414768..415100 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: O3Y_RS16130
• Protein ID: WP_000071008.1
• Coordinates: 415087..415401 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O3Y_RS20520	410210..410413	+	204	WP_001911745.1	hypothetical protein	-
O3Y_RS20325	410694..410867	+	174	WP_001882305.1	DUF3709 domain-containing protein	-
O3Y_RS16100	411227..411496	+	270	WP_001198131.1	hypothetical protein	-
O3Y_RS19855	411814..411985	+	172	Protein_432	DUF645 family protein	-
O3Y_RS16105	412194..412580	+	387	WP_000703163.1	VOC family protein	-
O3Y_RS16115	413194..413571	+	378	WP_000411109.1	hypothetical protein	-
O3Y_RS20630	413628..413742	+	115	Protein_435	acetyltransferase	-
O3Y_RS19860	413691..413972	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
O3Y_RS19875	414138..414251	+	114	WP_001889158.1	hypothetical protein	-
O3Y_RS19880	414200..414427	+	228	Protein_438	DUF1289 domain-containing protein	-
O3Y_RS16125	414768..415100	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
O3Y_RS16130	415087..415401	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
O3Y_RS16135	415538..415972	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
O3Y_RS20420	416140..416295	+	156	WP_000751734.1	hypothetical protein	-
O3Y_RS16145	416420..417400	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
O3Y_RS16150	417468..417774	+	307	Protein_444	CatB-related O-acetyltransferase	-
O3Y_RS16155	417911..418603	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
O3Y_RS16160	418603..419004	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
O3Y_RS20335	418974..419117	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
O3Y_RS16165	419192..419605	+	414	WP_000049417.1	VOC family protein	-
O3Y_RS16170	419819..420070	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
O3Y_RS16175	420060..420347	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304637..435582	130945
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	309717..435206	125489
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	416420..417400	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T26152 WP_000843587.1 NC_016945:414768-415100 [Vibrio cholerae IEC224]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T26152 NC_016945:414768-415100 [Vibrio cholerae IEC224]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT26152 WP_000071008.1 NC_016945:415087-415401 [Vibrio cholerae IEC224]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT26152 NC_016945:415087-415401 [Vibrio cholerae IEC224]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA