Detailed information of TA system TA046756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 95732..96347
• Replicon: chromosome
• Accession: NZ_CP007635
• Organism: Vibrio cholerae strain 2012EL-2176

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: EN18_RS14755
• Protein ID: WP_001162670.1
• Coordinates: 95732..96019 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EN18_RS14760
• Protein ID: WP_001232701.1
• Coordinates: 96030..96347 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EN18_RS21555	91368..91463	+	96	WP_001907607.1	DUF645 family protein	-
EN18_RS14725	91657..91974	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EN18_RS14730	91993..92235	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EN18_RS14735	92477..92986	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
EN18_RS14740	93043..93696	+	654	WP_000226874.1	hypothetical protein	-
EN18_RS14745	94006..94224	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
EN18_RS20640	94627..94860	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
EN18_RS14750	95285..95465	+	181	Protein_140	DUF645 family protein	-
EN18_RS14755	95732..96019	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EN18_RS14760	96030..96347	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
EN18_RS21560	96344..96454	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
EN18_RS21565	96631..96756	+	126	WP_001944767.1	DUF645 family protein	-
EN18_RS21570	96772..96810	+	39	WP_106019119.1	hypothetical protein	-
EN18_RS14770	97023..98027	+	1005	WP_000964925.1	LD-carboxypeptidase	-
EN18_RS14775	98180..98626	+	447	WP_000128977.1	hypothetical protein	-
EN18_RS14780	98746..99675	+	930	WP_000335802.1	hypothetical protein	-
EN18_RS21185	99672..99782	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
EN18_RS14785	99810..100286	+	477	WP_001047180.1	GNAT family N-acetyltransferase	-
EN18_RS14790	100423..100938	+	516	WP_001201528.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31037..137542	106505
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	36117..137166	101049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T46756 WP_001162670.1 NZ_CP007635:95732-96019 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T46756 NZ_CP007635:95732-96019 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT46756 WP_001232701.1 NZ_CP007635:96030-96347 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT46756 NZ_CP007635:96030-96347 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36