Detailed information of TA system TA095385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 118563..119656
• Replicon: chromosome
• Accession: NZ_CP026648
• Organism: Vibrio cholerae O1 biovar El Tor strain HC1037

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: C4E16_RS15155
• Protein ID: WP_000351248.1
• Coordinates: 119255..119656 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: C4E16_RS15150
• Protein ID: WP_001047169.1
• Coordinates: 118563..119255 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4E16_RS15085	113846..114223	+	378	WP_000411109.1	hypothetical protein	-
C4E16_RS19580	114280..114394	+	115	Protein_173	acetyltransferase	-
C4E16_RS15090	114343..114624	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
C4E16_RS15105	114790..114903	+	114	WP_001889158.1	hypothetical protein	-
C4E16_RS15110	114852..115079	+	228	WP_073426587.1	DUF1289 domain-containing protein	-
C4E16_RS15125	115420..115752	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C4E16_RS15130	115739..116053	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
C4E16_RS15135	116190..116624	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
C4E16_RS19445	116792..116947	+	156	WP_000751734.1	hypothetical protein	-
C4E16_RS15140	117072..118052	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
C4E16_RS15145	118120..118426	+	307	Protein_182	CatB-related O-acetyltransferase	-
C4E16_RS15150	118563..119255	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
C4E16_RS15155	119255..119656	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C4E16_RS15160	119626..119769	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
C4E16_RS15165	119844..120257	+	414	WP_000049417.1	VOC family protein	-
C4E16_RS15170	120471..120722	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
C4E16_RS15175	120712..120999	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C4E16_RS15180	121127..121582	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
C4E16_RS15185	121904..122056	+	153	WP_001884520.1	DUF645 family protein	-
C4E16_RS15195	122258..122755	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
C4E16_RS15200	122752..123024	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
C4E16_RS15205	123255..123923	+	669	WP_000043871.1	hypothetical protein	-
C4E16_RS15210	124075..124362	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31043..136234	105191
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	36123..135858	99735
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	117072..118052	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T95385 WP_000351248.1 NZ_CP026648:119255-119656 [Vibrio cholerae O1 biovar El Tor]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T95385 NZ_CP026648:119255-119656 [Vibrio cholerae O1 biovar El Tor]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT95385 WP_001047169.1 NZ_CP026648:118563-119255 [Vibrio cholerae O1 biovar El Tor]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT95385 NZ_CP026648:118563-119255 [Vibrio cholerae O1 biovar El Tor]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34