Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 101074..101602 Replicon chromosome
Accession NZ_CP007635
Organism Vibrio cholerae strain 2012EL-2176

Toxin (Protein)


Gene name relE Uniprot ID H9L4R3
Locus tag EN18_RS14795 Protein ID WP_000221352.1
Coordinates 101074..101364 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID H9L4T4
Locus tag EN18_RS14800 Protein ID WP_000213183.1
Coordinates 101354..101602 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EN18_RS21560 96344..96454 + 111 WP_134814058.1 DUF3265 domain-containing protein -
EN18_RS21565 96631..96756 + 126 WP_001944767.1 DUF645 family protein -
EN18_RS21570 96772..96810 + 39 WP_106019119.1 hypothetical protein -
EN18_RS14770 97023..98027 + 1005 WP_000964925.1 LD-carboxypeptidase -
EN18_RS14775 98180..98626 + 447 WP_000128977.1 hypothetical protein -
EN18_RS14780 98746..99675 + 930 WP_000335802.1 hypothetical protein -
EN18_RS21185 99672..99782 + 111 WP_071917832.1 DUF3265 domain-containing protein -
EN18_RS14785 99810..100286 + 477 WP_001047180.1 GNAT family N-acetyltransferase -
EN18_RS14790 100423..100938 + 516 WP_001201528.1 lipocalin family protein -
EN18_RS14795 101074..101364 - 291 WP_000221352.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EN18_RS14800 101354..101602 - 249 WP_000213183.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EN18_RS14815 102173..102826 + 654 WP_000036957.1 site-specific DNA-methyltransferase -
EN18_RS14820 103098..103523 + 426 WP_000415748.1 hypothetical protein -
EN18_RS14825 103731..104126 + 396 WP_000649527.1 DUF3465 domain-containing protein -
EN18_RS14830 104277..104807 + 531 WP_000415563.1 hypothetical protein -
EN18_RS14835 104945..105445 + 501 WP_000789723.1 hypothetical protein -
EN18_RS14840 105601..105999 + 399 WP_001882293.1 hypothetical protein -
EN18_RS14845 106099..106563 - 465 WP_000140244.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 31037..137542 106505
inside Integron - - 36117..137166 101049


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11247.23 Da        Isoelectric Point: 10.4678

>T46757 WP_000221352.1 NZ_CP007635:c101364-101074 [Vibrio cholerae]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Download         Length: 291 bp

>T46757 NZ_CP007635:c101364-101074 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8937.28 Da        Isoelectric Point: 4.1079

>AT46757 WP_000213183.1 NZ_CP007635:c101602-101354 [Vibrio cholerae]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Download         Length: 249 bp

>AT46757 NZ_CP007635:c101602-101354 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H9L4R3

Antitoxin

Source ID Structure
AlphaFold DB A0A366A3W8

References