Detailed information of TA system TA145850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 372954..373569
• Replicon: chromosome
• Accession: NZ_CP047300
• Organism: Vibrio cholerae O1 biovar El Tor strain P27459

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: GTF71_RS15300
• Protein ID: WP_001162670.1
• Coordinates: 372954..373241 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GTF71_RS15305
• Protein ID: WP_001232701.1
• Coordinates: 373252..373569 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF71_RS15260	368590..368685	+	96	WP_001907607.1	DUF645 family protein	-
GTF71_RS15265	368879..369196	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GTF71_RS15270	369215..369457	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GTF71_RS15275	369699..370208	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
GTF71_RS15280	370265..370918	+	654	WP_000226874.1	hypothetical protein	-
GTF71_RS15285	371228..371446	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
GTF71_RS15290	371849..372082	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
GTF71_RS15295	372507..372687	+	181	Protein_369	DUF645 family protein	-
GTF71_RS15300	372954..373241	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTF71_RS15305	373252..373569	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
GTF71_RS15310	373566..373676	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
GTF71_RS15315	373853..373978	+	126	WP_001944767.1	DUF645 family protein	-
GTF71_RS15320	373994..374032	+	39	WP_106019119.1	hypothetical protein	-
GTF71_RS15325	374256..374996	+	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
GTF71_RS15330	375201..376031	+	831	WP_000056616.1	abortive infection family protein	-
GTF71_RS15335	376088..376570	+	483	WP_001895003.1	hypothetical protein	-
GTF71_RS15340	377017..377892	+	876	WP_000259044.1	hypothetical protein	-
GTF71_RS15345	378022..378477	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	306926..439292	132366
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	312006..438916	126910

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T145850 WP_001162670.1 NZ_CP047300:372954-373241 [Vibrio cholerae O1 biovar El Tor]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T145850 NZ_CP062702:3611484-3612140 [Escherichia coli O157:H7]
ATGGCTTATGATTACAGCGCCAGCCTCAACCCGCAAAAAGCTCTGATCTGGCGTATCGTCCATCGGGACAATATCCCGTG
GATTCTGGATAACGGGCTGCACTGCGGCAACAGTCTGGTGCAGGCGGAAAACTGGATCAATATCGGCAACCCGGAGCTGA
TCGGCAAGCGTGCCGGGCATCCGGTACCTGTCGGAACGGGCGGGACACTGCATGACTATGTGCCGTTTTATTTTACCCCC
TTCTCGCCAATGCTGATGAACATCCACAGCGGGCGCGGCGGCATTAAGCAACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGCAACTTGCGAAACGTGGCAGCACACGACGTACCCTTCGTGTTTACCGACAGCCATGCTTACTATAACTGGACCAACT
ACTACACCAGTCTGGATAGTCTGGACCAGATCGACTGGCCCATTTTGCAGGCACGGGACTTTCGCCGCGATCCAGACGAT
CCGGCTAAATTTGAACGCTATCAGGCAGAGGCGCTAATCTGGCAACACTGCCCGATCTCTCTGCTGGATGGGATAATTTG
TTACAGCGAAGAGGTCAGGTTACAATTAGAACAATGGCTGTTTCAGCGTAATCTAACAATGTCCGTTCACACACGGGCTG
GATGGTACTTCTCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT145850 WP_001232701.1 NZ_CP047300:373252-373569 [Vibrio cholerae O1 biovar El Tor]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT145850 NZ_CP062702:3612137-3613204 [Escherichia coli O157:H7]
ATGATCACATATACACAAGGCAATTTACTGGACGCACCGGTGGAAGCTCTGGTGAATACGGTTAATACCGTCGGTGTCAT
GGGGAAAGGCATCGCGTTGATGTTTAAAGAGCGCTTCCCCGAAAATATGAAAGTCTATGCCCTCGCCTGCAAACAAAAGC
AGGTGATCACCGGCAAAATGTTCATTACCGAAACCGGGGAACTGATGGGACCGCGCTGGATTGTTAATTTTCCGACCAAA
CAGCACTGGCGCGCTGATTCCCGCATGGAGTGGATTGAAGATGGCTTGCAGGACTTACGCCGCTTTTTGATTGAAGAGAA
CGTGCAGTCCATCGCCATTCCACCACTGGGCGCAGGCAACGGTGGTTTGAACTGGCCGGACGTTCGGGCGCAGATTGAAT
CTGCCCTTGGCGACCTTCAGGACGTTGATATCCTGATTTACCAACCGACTGAAAAATACCAGAACGTTGCCAAAAGCACT
GGCGTAAAAAAGCTCACTCCCGCCAGAGCCGCCATCGCTGAACTGGTGCGTCGCTACTGGGTATTGGGGATGGAGTGCAG
CCTACTGGAAATCCAGAAACTGGCCTGGCTGTTACAACGCGCCATTGAGCAGCATCAACAGGACGATATCCTCAAACTGC
GGTTTGAAGCTCACTATTATGGCCCCTATGCGCCCAATCTGAACCATCTGCTTAATGCGCTGGACGGCACCTATCTTAAA
GCCGAGAAACGCATCCCGGACAGCCAACCGCTGGATGTGATCTGGTTTAACGATCAGAAAAAAGAACACGTTAATGCGTA
TCTGAACAACGAAGCCAGAGAGTGGCTACCTGCGCTGGAGCAGGTCAGCCAGTTAATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGTTGCTGGCAACCGTAGACTGGTTGCTGAGCCGTGGCGAATGTCAACCAACGCTGGATTCCGTTAAGGAAGGG
CTGCACCAGTGGCCTGCGGGCGAACGCTGGGCCAGCCGTAAGTTGAGGCTGTTTGATAATAATAATCTGCAATTTGCCAT
TAACCGCGTAATGGAGTTCCATTGCTGA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36