Detailed information of TA system TA058230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 455310..456076
• Replicon: chromosome
• Accession: NZ_CP013302
• Organism: Vibrio cholerae strain C5

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: ASZ80_RS17015
• Protein ID: WP_000982260.1
• Coordinates: 455310..455807 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2J6
• Locus tag: ASZ80_RS17020
• Protein ID: WP_000246253.1
• Coordinates: 455804..456076 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ80_RS16965	451172..451478	+	307	Protein_502	CatB-related O-acetyltransferase	-
ASZ80_RS16970	451615..452307	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
ASZ80_RS16975	452307..452708	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
ASZ80_RS20690	452678..452821	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
ASZ80_RS16985	452896..453309	+	414	WP_000049417.1	VOC family protein	-
ASZ80_RS16990	453523..453774	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ80_RS16995	453764..454051	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ80_RS17000	454179..454634	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ80_RS17010	454956..455108	+	153	WP_001884520.1	DUF645 family protein	-
ASZ80_RS17015	455310..455807	-	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
ASZ80_RS17020	455804..456076	-	273	WP_000246253.1	DUF1778 domain-containing protein	Antitoxin
ASZ80_RS17025	456307..456975	+	669	WP_000043871.1	hypothetical protein	-
ASZ80_RS17030	457127..457414	+	288	WP_000426470.1	hypothetical protein	-
ASZ80_RS17035	457555..457926	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ80_RS17040	457993..458418	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
ASZ80_RS17045	458415..458948	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
ASZ80_RS17050	459150..459407	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ80_RS17055	459395..459697	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ80_RS17060	459931..460848	+	918	WP_000186333.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304694..469286	164592
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309774..468910	159136
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	450124..451104	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T58230 WP_000982260.1 NZ_CP013302:c455807-455310 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T58230 NZ_CP013302:c455807-455310 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9683.22 Da
• Isoelectric Point: 6.2988

>AT58230 WP_000246253.1 NZ_CP013302:c456076-455804 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT58230 NZ_CP013302:c456076-455804 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1

Antitoxin

• AlphaFold DB: A0A0F2IC79