Detailed information of TA system TA023713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 390643..391276
• Replicon: chromosome II
• Accession: NC_012580
• Organism: Vibrio cholerae M66-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: VCM66_RS15290
• Protein ID: WP_000843587.1
• Coordinates: 390643..390975 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: VCM66_RS15295
• Protein ID: WP_000071008.1
• Coordinates: 390962..391276 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VCM66_RS19570	386084..386287	+	204	WP_001911745.1	hypothetical protein	-
VCM66_RS19410	386568..386741	+	174	WP_001882305.1	DUF3709 domain-containing protein	-
VCM66_RS15265	387101..387370	+	270	WP_001198131.1	hypothetical protein	-
VCM66_RS19610	387688..387859	+	172	Protein_388	DUF645 family protein	-
VCM66_RS15270	388068..388454	+	387	WP_000703163.1	VOC family protein	-
VCM66_RS15275	388656..388898	+	243	WP_000107461.1	hypothetical protein	-
VCM66_RS15280	389069..389446	+	378	WP_000411109.1	hypothetical protein	-
VCM66_RS19585	389503..389617	+	115	Protein_392	acetyltransferase	-
VCM66_RS18970	389566..389847	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
VCM66_RS18985	390013..390126	+	114	WP_001889158.1	hypothetical protein	-
VCM66_RS18990	390075..390302	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
VCM66_RS15290	390643..390975	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VCM66_RS15295	390962..391276	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
VCM66_RS15300	391413..391847	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
VCM66_RS19590	392015..392170	+	156	WP_000751734.1	hypothetical protein	-
VCM66_RS15310	392295..393275	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
VCM66_RS15315	393343..393615	+	273	Protein_401	CatB-related O-acetyltransferase	-
VCM66_RS15320	393786..394478	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
VCM66_RS15325	394478..394879	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
VCM66_RS19425	394849..394992	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
VCM66_RS15330	395067..395480	+	414	WP_000049417.1	VOC family protein	-
VCM66_RS15335	395694..395945	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VCM66_RS15340	395935..396222	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	305869..409778	103909
	-	inside	Integron	-	-	310949..409402	98453
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	392295..393275	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T23713 WP_000843587.1 NC_012580:390643-390975 [Vibrio cholerae M66-2]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T23713 NC_012580:390643-390975 [Vibrio cholerae M66-2]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT23713 WP_000071008.1 NC_012580:390962-391276 [Vibrio cholerae M66-2]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT23713 NC_012580:390962-391276 [Vibrio cholerae M66-2]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA