Detailed information of TA system TA145886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 334939..335437
• Replicon: chromosome
• Accession: NZ_CP047304
• Organism: Vibrio cholerae O1 biovar El Tor strain E7946

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: GTF74_RS15295
• Protein ID: WP_000589156.1
• Coordinates: 334939..335205 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: GTF74_RS15300
• Protein ID: WP_000643598.1
• Coordinates: 335192..335437 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF74_RS15240	330360..330566	+	207	Protein_294	DUF3709 domain-containing protein	-
GTF74_RS15245	330766..330867	+	102	WP_001921603.1	hypothetical protein	-
GTF74_RS15250	330857..331243	+	387	WP_000703163.1	VOC family protein	-
GTF74_RS15255	331438..331689	+	252	WP_001890111.1	TIGR03643 family protein	-
GTF74_RS15260	331662..331811	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
GTF74_RS15265	331903..332145	+	243	WP_000107461.1	hypothetical protein	-
GTF74_RS15270	332397..332675	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
GTF74_RS15275	333050..333193	+	144	Protein_301	DUF645 family protein	-
GTF74_RS15280	333247..333285	+	39	WP_082798268.1	hypothetical protein	-
GTF74_RS15285	333497..333964	+	468	WP_001289288.1	OsmC family protein	-
GTF74_RS15290	334092..334754	+	663	WP_000960654.1	hypothetical protein	-
GTF74_RS15295	334939..335205	+	267	WP_000589156.1	BrnT family toxin	Toxin
GTF74_RS15300	335192..335437	+	246	WP_000643598.1	hypothetical protein	Antitoxin
GTF74_RS15305	335533..336327	+	795	WP_001911581.1	hypothetical protein	-
GTF74_RS15310	336430..336558	+	129	WP_071908338.1	general secretion pathway protein GspI	-
GTF74_RS15315	336619..336801	+	183	WP_000923182.1	DUF645 family protein	-
GTF74_RS15320	337017..338021	+	1005	WP_000964920.1	LD-carboxypeptidase	-
GTF74_RS15325	338174..338533	+	360	WP_001071541.1	VOC family protein	-
GTF74_RS15330	338806..339039	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
GTF74_RS15335	339307..339813	+	507	WP_000393074.1	hypothetical protein	-
GTF74_RS15340	339970..340356	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	307032..436751	129719
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	312112..436375	124263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T145886 WP_000589156.1 NZ_CP047304:334939-335205 [Vibrio cholerae O1 biovar El Tor]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T145886 NZ_CP062705:c4963695-4963345 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT145886 WP_000643598.1 NZ_CP047304:335192-335437 [Vibrio cholerae O1 biovar El Tor]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT145886 NZ_CP062705:c4963940-4963689 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2