74624

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 814810..815425 Replicon chromosome
Accession NZ_CP020407
Organism Vibrio cholerae strain FDAARGOS_223

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag A6J62_RS04045 Protein ID WP_001162670.1
Coordinates 815138..815425 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag A6J62_RS04040 Protein ID WP_001232701.1
Coordinates 814810..815127 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6J62_RS03990 809902..810357 - 456 WP_001245327.1 GNAT family N-acetyltransferase -
A6J62_RS03995 810487..811362 - 876 WP_000259044.1 hypothetical protein -
A6J62_RS04005 811809..812291 - 483 WP_001895003.1 hypothetical protein -
A6J62_RS04010 812348..813178 - 831 WP_000056616.1 abortive infection family protein -
A6J62_RS04015 813383..814123 - 741 WP_000469836.1 PhzF family phenazine biosynthesis protein -
A6J62_RS19570 814347..814385 - 39 WP_106019119.1 hypothetical protein -
A6J62_RS04025 814401..814526 - 126 WP_001944767.1 DUF645 family protein -
A6J62_RS19670 814703..814813 - 111 WP_134814058.1 DUF3265 domain-containing protein -
A6J62_RS04040 814810..815127 - 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
A6J62_RS04045 815138..815425 - 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A6J62_RS04050 815692..815872 - 181 Protein_750 DUF645 family protein -
A6J62_RS04055 816297..816530 - 234 WP_032482776.1 DUF3709 domain-containing protein -
A6J62_RS04060 816933..817151 - 219 WP_001917086.1 DUF3709 domain-containing protein -
A6J62_RS04065 817461..818114 - 654 WP_000226874.1 hypothetical protein -
A6J62_RS04070 818171..818680 - 510 WP_000405987.1 GNAT family N-acetyltransferase -
A6J62_RS04075 818922..819164 + 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
A6J62_RS04080 819183..819500 + 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
A6J62_RS19675 819694..819789 - 96 WP_001907607.1 DUF645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 749087..881909 132822
inside Integron - - 749463..875103 125640


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T74624 WP_001162670.1 NZ_CP020407:c815425-815138 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T74624 NZ_CP020407:c815425-815138 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT74624 WP_001232701.1 NZ_CP020407:c815127-814810 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT74624 NZ_CP020407:c815127-814810 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36

Antitoxin

Source ID Structure

References