Detailed information of TA system TA057708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 849487..850580
• Replicon: chromosome
• Accession: NZ_CP013013
• Organism: Vibrio cholerae strain Env-390

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: AP033_RS03880
• Protein ID: WP_000351248.1
• Coordinates: 849487..849888 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: AP033_RS03885
• Protein ID: WP_001047169.1
• Coordinates: 849888..850580 (-)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AP033_RS03835	844511..845491	-	981	WP_000019368.1	IS5-like element ISVch5 family transposase	-
AP033_RS03840	845561..845887	-	327	Protein_748	hypothetical protein	-
AP033_RS03845	846118..846390	+	273	WP_000246253.1	DUF1778 domain-containing protein	-
AP033_RS03850	846387..846884	+	498	WP_000982260.1	GNAT family N-acetyltransferase	-
AP033_RS03855	847087..847239	-	153	WP_001884520.1	DUF645 family protein	-
AP033_RS03860	847561..848016	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
AP033_RS03865	848144..848431	-	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AP033_RS03870	848421..848672	-	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AP033_RS03875	848886..849299	-	414	WP_000049417.1	VOC family protein	-
AP033_RS19795	849374..849517	-	144	WP_071908341.1	DUF3265 domain-containing protein	-
AP033_RS03880	849487..849888	-	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AP033_RS03885	849888..850580	-	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
AP033_RS03890	850751..851023	-	273	Protein_759	CatB-related O-acetyltransferase	-
AP033_RS03895	851091..852071	+	981	WP_000019368.1	IS5-like element ISVch5 family transposase	-
AP033_RS03900	852196..852396	-	201	WP_001198130.1	hypothetical protein	-
AP033_RS19800	852756..852929	-	174	WP_001882305.1	DUF3709 domain-containing protein	-
AP033_RS03905	853222..853773	-	552	WP_000821930.1	hypothetical protein	-
AP033_RS03910	853929..854786	-	858	WP_001279526.1	DUF2806 domain-containing protein	-
AP033_RS03915	854933..855547	-	615	WP_000789577.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0.001	flank	IS/Tn	-	-	844511..845491	980
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0.001	flank	IS/Tn	-	-	851091..852071	980
	-	inside	Integron	-	-	829699..942463	112764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T57708 WP_000351248.1 NZ_CP013013:c849888-849487 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T57708 NZ_CP013013:c849888-849487 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT57708 WP_001047169.1 NZ_CP013013:c850580-849888 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT57708 NZ_CP013013:c850580-849888 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34