Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-RelB
Location 434023..434582 Replicon chromosome
Accession NZ_CP047304
Organism Vibrio cholerae O1 biovar El Tor strain E7946

Toxin (Protein)


Gene name relE Uniprot ID Q9K2M8
Locus tag GTF74_RS16180 Protein ID WP_000578476.1
Coordinates 434023..434301 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9L991
Locus tag GTF74_RS16185 Protein ID WP_000381183.1
Coordinates 434298..434582 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTF74_RS16135 429323..429760 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
GTF74_RS16140 429824..430042 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
GTF74_RS16145 430217..431089 + 873 WP_000254716.1 DUF3800 domain-containing protein -
GTF74_RS16150 431239..431838 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
GTF74_RS16155 431895..431999 + 105 WP_099607150.1 acetyltransferase -
GTF74_RS16160 432030..432299 + 270 WP_001114075.1 DUF4160 domain-containing protein -
GTF74_RS16165 432293..432814 + 522 WP_000921691.1 DUF2442 domain-containing protein -
GTF74_RS16170 433113..433238 + 126 WP_001905700.1 DUF645 family protein -
GTF74_RS16175 433489..433884 + 396 WP_001000867.1 hypothetical protein -
GTF74_RS16180 434023..434301 - 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
GTF74_RS16185 434298..434582 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
GTF74_RS16190 434776..435291 + 516 WP_000343779.1 GNAT family N-acetyltransferase -
GTF74_RS16195 435348..435443 + 96 WP_099607151.1 acetyltransferase -
GTF74_RS16200 435475..435888 + 414 WP_000049420.1 VOC family protein -
GTF74_RS16205 435895..436027 - 133 Protein_487 hypothetical protein -
GTF74_RS16210 436443..437620 + 1178 WP_086010879.1 IS3-like element ISVch4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 307032..436751 129719
inside Integron - - 312112..436375 124263
flank IS/Tn - - 429323..429760 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(2-89)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-70)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10819.59 Da        Isoelectric Point: 5.1651

>T145901 WP_000578476.1 NZ_CP047304:c434301-434023 [Vibrio cholerae O1 biovar El Tor]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Download         Length: 279 bp

>T145901 NZ_CP062708:1094580-1094915 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10901.25 Da        Isoelectric Point: 8.0775

>AT145901 WP_000381183.1 NZ_CP047304:c434582-434298 [Vibrio cholerae O1 biovar El Tor]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Download         Length: 285 bp

>AT145901 NZ_CP062708:1094332-1094580 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9K2M8

Antitoxin

Source ID Structure
AlphaFold DB A0A067BL33

References