46750

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 50881..51647 Replicon chromosome
Accession NZ_CP007635
Organism Vibrio cholerae strain 2012EL-2176

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag EN18_RS14405 Protein ID WP_000982260.1
Coordinates 50881..51378 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag EN18_RS14410 Protein ID WP_000246253.1
Coordinates 51375..51647 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EN18_RS14360 46323..47027 + 705 WP_000087610.1 HNH endonuclease -
EN18_RS14365 47185..47523 + 339 WP_000713853.1 hypothetical protein -
EN18_RS14370 47677..47994 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
EN18_RS14375 48013..48255 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
EN18_RS14380 48499..48885 + 387 WP_001015867.1 NUDIX hydrolase -
EN18_RS20495 48942..49055 + 114 WP_001900214.1 hypothetical protein -
EN18_RS14385 49004..49285 + 282 WP_001894429.1 DUF1289 domain-containing protein -
EN18_RS14395 49543..50079 + 537 WP_000469482.1 GNAT family N-acetyltransferase -
EN18_RS14400 50216..50731 + 516 WP_001201509.1 lipocalin family protein -
EN18_RS14405 50881..51378 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
EN18_RS14410 51375..51647 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
EN18_RS21085 52040..52165 + 126 WP_001900195.1 DUF645 family protein -
EN18_RS21090 52181..52219 + 39 WP_106019118.1 hypothetical protein -
EN18_RS14425 52415..52861 + 447 WP_000006157.1 hypothetical protein -
EN18_RS14430 52924..54135 + 1212 WP_001122813.1 IS256 family transposase -
EN18_RS14435 54322..54591 - 270 WP_000277238.1 type II toxin-antitoxin system YafQ family toxin -
EN18_RS14440 54584..54862 - 279 WP_001258569.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
EN18_RS20510 55148..55426 + 279 WP_001921601.1 DUF3709 domain-containing protein -
EN18_RS21105 55678..55884 + 207 Protein_69 DUF3709 domain-containing protein -
EN18_RS21110 56084..56185 + 102 WP_001921603.1 hypothetical protein -
EN18_RS14450 56175..56561 + 387 WP_000703163.1 VOC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 31037..137542 106505
inside Integron - - 36117..137166 101049
- flank IS/Tn - - 52924..54135 1211


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T46750 WP_000982260.1 NZ_CP007635:c51378-50881 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T46750 NZ_CP007635:c51378-50881 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT46750 WP_000246253.1 NZ_CP007635:c51647-51375 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT46750 NZ_CP007635:c51647-51375 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References