Detailed information of TA system TA095388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 124941..125896
• Replicon: chromosome
• Accession: NZ_CP026648
• Organism: Vibrio cholerae O1 biovar El Tor strain HC1037

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: C4E16_RS15225
• Protein ID: WP_000118351.1
• Coordinates: 125363..125896 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: C4E16_RS19585
• Protein ID: WP_001882332.1
• Coordinates: 124941..125366 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4E16_RS15170	120471..120722	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
C4E16_RS15175	120712..120999	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C4E16_RS15180	121127..121582	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
C4E16_RS15185	121904..122056	+	153	WP_001884520.1	DUF645 family protein	-
C4E16_RS15195	122258..122755	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
C4E16_RS15200	122752..123024	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
C4E16_RS15205	123255..123923	+	669	WP_000043871.1	hypothetical protein	-
C4E16_RS15210	124075..124362	+	288	WP_000426470.1	hypothetical protein	-
C4E16_RS15215	124503..124874	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
C4E16_RS19585	124941..125366	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
C4E16_RS15225	125363..125896	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
C4E16_RS15235	126098..126355	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
C4E16_RS15240	126343..126645	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C4E16_RS15250	126879..127796	+	918	WP_000186333.1	alpha/beta hydrolase	-
C4E16_RS15255	127953..128342	+	390	WP_001081302.1	hypothetical protein	-
C4E16_RS15260	128478..128687	+	210	Protein_202	GNAT family N-acetyltransferase	-
C4E16_RS15265	128806..129243	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
C4E16_RS15270	129307..129525	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
C4E16_RS15275	129700..130572	+	873	WP_000254716.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31043..136234	105191
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	36123..135858	99735
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	128806..129243	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T95388 WP_000118351.1 NZ_CP026648:125363-125896 [Vibrio cholerae O1 biovar El Tor]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T95388 NZ_CP026648:125363-125896 [Vibrio cholerae O1 biovar El Tor]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT95388 WP_001882332.1 NZ_CP026648:124941-125366 [Vibrio cholerae O1 biovar El Tor]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT95388 NZ_CP026648:124941-125366 [Vibrio cholerae O1 biovar El Tor]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94