Detailed information of TA system TA058234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/-
• Location: 464565..465349
• Replicon: chromosome
• Accession: NZ_CP013302
• Organism: Vibrio cholerae strain C5

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A086SLD6
• Locus tag: ASZ80_RS17105
• Protein ID: WP_001114075.1
• Coordinates: 464565..464834 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: Q9KM84
• Locus tag: ASZ80_RS17110
• Protein ID: WP_000921691.1
• Coordinates: 464828..465349 (+)
• Length: 174 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ80_RS17060	459931..460848	+	918	WP_000186333.1	alpha/beta hydrolase	-
ASZ80_RS17070	461005..461394	+	390	WP_001081302.1	hypothetical protein	-
ASZ80_RS17075	461530..461739	+	210	Protein_522	GNAT family N-acetyltransferase	-
ASZ80_RS17080	461858..462295	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
ASZ80_RS17085	462359..462577	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
ASZ80_RS17095	462752..463624	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
ASZ80_RS17100	463774..464373	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
ASZ80_RS20705	464430..464534	+	105	WP_099607150.1	acetyltransferase	-
ASZ80_RS17105	464565..464834	+	270	WP_001114075.1	DUF4160 domain-containing protein	Toxin
ASZ80_RS17110	464828..465349	+	522	WP_000921691.1	DUF2442 domain-containing protein	Antitoxin
ASZ80_RS20710	465648..465773	+	126	WP_001905700.1	DUF645 family protein	-
ASZ80_RS17125	466024..466419	+	396	WP_001000867.1	hypothetical protein	-
ASZ80_RS17130	466558..466836	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
ASZ80_RS17135	466833..467117	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
ASZ80_RS17140	467311..467826	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
ASZ80_RS17145	468010..468423	+	414	WP_000049420.1	VOC family protein	-
ASZ80_RS17160	468978..470155	+	1178	WP_086010879.1	IS3-like element ISVch4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304694..469286	164592
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309774..468910	159136
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	461858..462295	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Protein of unknown function DUF2442 (4-59)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.94 Da
• Isoelectric Point: 6.6303

>T58234 WP_001114075.1 NZ_CP013302:464565-464834 [Vibrio cholerae]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Nucleotide

• Length: 270 bp

>T58234 NZ_CP013302:464565-464834 [Vibrio cholerae]
ATGCCAGAGATTGATGCCCTATTAGGTCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTACCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AACGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin

Protein

• Length: 174 a.a.
• Molecular weight: 19409.01 Da
• Isoelectric Point: 6.7342

>AT58234 WP_000921691.1 NZ_CP013302:464828-465349 [Vibrio cholerae]
MLKVIDVDFVSDHTLELTFNDGYQGYADLSVYFKKAPFSEIKDFKRFSLTRDGSLNWDGNELTAATLRDITKGSQKSVEL
SFNVQEMEAVIKQASWESMMEGRPDILQAAIRSYVEQFGHGQVIAKAGIKSRTSAYRSLKPETTPNFGTLVQLGHAVIEL
AKDRTVANKEPRI

Nucleotide

• Length: 522 bp

>AT58234 NZ_CP013302:464828-465349 [Vibrio cholerae]
ATGTTGAAAGTCATAGATGTTGATTTTGTCTCAGACCATACATTAGAACTGACGTTCAATGATGGTTACCAAGGCTATGC
CGACTTGTCGGTATACTTTAAAAAAGCACCGTTTTCAGAAATTAAGGACTTTAAACGCTTTTCCTTGACTCGTGATGGCT
CGTTAAATTGGGATGGTAATGAGTTAACGGCCGCGACACTCCGTGACATAACCAAGGGCTCACAAAAGTCGGTGGAGTTA
AGTTTTAATGTTCAAGAAATGGAAGCCGTCATCAAACAGGCCTCATGGGAATCGATGATGGAGGGGCGTCCCGACATCTT
GCAAGCGGCTATTCGTTCTTACGTAGAGCAGTTTGGTCATGGTCAAGTTATTGCAAAGGCGGGTATAAAAAGTCGTACGA
GTGCTTATCGCTCCTTGAAGCCAGAAACAACGCCAAACTTTGGCACACTAGTTCAGTTAGGGCACGCCGTCATCGAACTA
GCGAAAGATAGAACAGTGGCAAACAAGGAACCACGCATATAA

Structures

Toxin

• AlphaFold DB: A0A086SLD6

Antitoxin

• AlphaFold DB: Q9KM84