Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/RelE-MqsA |
| Location | 433613..434246 | Replicon | chromosome |
| Accession | NZ_CP013308 | ||
| Organism | Vibrio cholerae strain E506 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | Q9KMA6 |
| Locus tag | ASZ84_RS16645 | Protein ID | WP_000843587.1 |
| Coordinates | 433613..433945 (+) | Length | 111 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | ASZ84_RS16650 | Protein ID | WP_000071008.1 |
| Coordinates | 433932..434246 (+) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ASZ84_RS20350 | 429054..429257 | + | 204 | WP_001911745.1 | hypothetical protein | - |
| ASZ84_RS20135 | 429538..429711 | + | 174 | WP_001882305.1 | DUF3709 domain-containing protein | - |
| ASZ84_RS16585 | 430071..430340 | + | 270 | WP_001198131.1 | hypothetical protein | - |
| ASZ84_RS20520 | 430658..430829 | + | 172 | Protein_452 | DUF645 family protein | - |
| ASZ84_RS16600 | 431038..431424 | + | 387 | WP_000703163.1 | VOC family protein | - |
| ASZ84_RS16605 | 431626..431868 | + | 243 | WP_000107461.1 | hypothetical protein | - |
| ASZ84_RS16610 | 432039..432416 | + | 378 | WP_000411109.1 | hypothetical protein | - |
| ASZ84_RS20480 | 432473..432587 | + | 115 | Protein_456 | acetyltransferase | - |
| ASZ84_RS16615 | 432536..432817 | + | 282 | WP_001894453.1 | DUF1289 domain-containing protein | - |
| ASZ84_RS16630 | 432983..433096 | + | 114 | WP_001889158.1 | hypothetical protein | - |
| ASZ84_RS16635 | 433045..433272 | + | 228 | WP_001894457.1 | DUF1289 domain-containing protein | - |
| ASZ84_RS16645 | 433613..433945 | + | 333 | WP_000843587.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| ASZ84_RS16650 | 433932..434246 | + | 315 | WP_000071008.1 | DNA-binding transcriptional regulator | Antitoxin |
| ASZ84_RS16655 | 434383..434817 | + | 435 | WP_000256036.1 | GNAT family N-acetyltransferase | - |
| ASZ84_RS20250 | 434985..435140 | + | 156 | WP_000751734.1 | hypothetical protein | - |
| ASZ84_RS16670 | 435265..436245 | - | 981 | WP_000019370.1 | IS5-like element ISVch5 family transposase | - |
| ASZ84_RS16675 | 436313..436619 | + | 307 | Protein_465 | CatB-related O-acetyltransferase | - |
| ASZ84_RS16680 | 436756..437448 | + | 693 | WP_001047169.1 | GNAT family N-acetyltransferase | - |
| ASZ84_RS16685 | 437448..437849 | + | 402 | WP_000351248.1 | type II toxin-antitoxin system death-on-curing family toxin | - |
| ASZ84_RS20150 | 437819..437962 | + | 144 | WP_071908341.1 | DUF3265 domain-containing protein | - |
| ASZ84_RS16690 | 438037..438450 | + | 414 | WP_000049417.1 | VOC family protein | - |
| ASZ84_RS16695 | 438664..438915 | + | 252 | WP_001250179.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| ASZ84_RS16700 | 438905..439192 | + | 288 | WP_000221354.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 304088..451655 | 147567 | |
| - | inside | Integron | - | - | 309168..451279 | 142111 | |
| flank | IS/Tn | - | - | 435265..436245 | 980 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 111 a.a. Molecular weight: 13005.95 Da Isoelectric Point: 9.9244
>T58291 WP_000843587.1 NZ_CP013308:433613-433945 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS
Download Length: 333 bp
>T58291 NZ_CP013308:433613-433945 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA
Antitoxin
Download Length: 105 a.a. Molecular weight: 11696.20 Da Isoelectric Point: 6.7600
>AT58291 WP_000071008.1 NZ_CP013308:433932-434246 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL
Download Length: 315 bp
>AT58291 NZ_CP013308:433932-434246 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|