Detailed information of TA system TA058356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 367479..367977
• Replicon: chromosome
• Accession: NZ_CP013314
• Organism: Vibrio cholerae strain E9120

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: ASZ79_RS16055
• Protein ID: WP_000589156.1
• Coordinates: 367479..367745 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: ASZ79_RS16060
• Protein ID: WP_000643598.1
• Coordinates: 367732..367977 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ79_RS20575	362900..363106	+	207	Protein_347	DUF3709 domain-containing protein	-
ASZ79_RS20580	363306..363407	+	102	WP_001921603.1	hypothetical protein	-
ASZ79_RS16010	363397..363783	+	387	WP_000703163.1	VOC family protein	-
ASZ79_RS16015	363978..364229	+	252	WP_001890111.1	TIGR03643 family protein	-
ASZ79_RS16020	364202..364351	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
ASZ79_RS16025	364443..364685	+	243	WP_000107461.1	hypothetical protein	-
ASZ79_RS16030	364937..365215	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
ASZ79_RS20990	365590..365733	+	144	Protein_354	DUF645 family protein	-
ASZ79_RS20995	365787..365825	+	39	WP_082798268.1	hypothetical protein	-
ASZ79_RS16045	366037..366504	+	468	WP_001289288.1	OsmC family protein	-
ASZ79_RS16050	366632..367294	+	663	WP_000960654.1	hypothetical protein	-
ASZ79_RS16055	367479..367745	+	267	WP_000589156.1	BrnT family toxin	Toxin
ASZ79_RS16060	367732..367977	+	246	WP_000643598.1	hypothetical protein	Antitoxin
ASZ79_RS16065	368073..368867	+	795	WP_001911581.1	hypothetical protein	-
ASZ79_RS21000	368970..369098	+	129	WP_071908338.1	general secretion pathway protein GspI	-
ASZ79_RS16080	369159..369341	+	183	WP_000923182.1	DUF645 family protein	-
ASZ79_RS16085	369557..370561	+	1005	WP_000964920.1	LD-carboxypeptidase	-
ASZ79_RS16090	370714..371073	+	360	WP_001071541.1	VOC family protein	-
ASZ79_RS16095	371346..371579	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
ASZ79_RS16100	371847..372353	+	507	WP_000393074.1	hypothetical protein	-
ASZ79_RS16105	372510..372896	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304700..468464	163764
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309780..468088	158308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T58356 WP_000589156.1 NZ_CP013314:367479-367745 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T58356 NZ_CP013314:367479-367745 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT58356 WP_000643598.1 NZ_CP013314:367732-367977 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT58356 NZ_CP013314:367732-367977 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2