Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 827771..828299 Replicon chromosome
Accession NZ_CP013312
Organism Vibrio cholerae strain E1320

Toxin (Protein)


Gene name relE Uniprot ID A0A0X1KZS6
Locus tag ASZ88_RS18460 Protein ID WP_000221354.1
Coordinates 828012..828299 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0X1KZS8
Locus tag ASZ88_RS18455 Protein ID WP_001250179.1
Coordinates 827771..828022 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ88_RS18405 823039..823353 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
ASZ88_RS18410 823490..823924 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
ASZ88_RS20985 824092..824247 + 156 WP_000751734.1 hypothetical protein -
ASZ88_RS18425 824372..825352 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
ASZ88_RS18430 825420..825726 + 307 Protein_835 CatB-related O-acetyltransferase -
ASZ88_RS18435 825863..826555 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
ASZ88_RS18440 826555..826956 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
ASZ88_RS20885 826926..827069 + 144 WP_071908341.1 DUF3265 domain-containing protein -
ASZ88_RS18450 827144..827557 + 414 WP_000049417.1 VOC family protein -
ASZ88_RS18455 827771..828022 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ASZ88_RS18460 828012..828299 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ASZ88_RS18465 828427..828882 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
ASZ88_RS18475 829204..829356 + 153 WP_001884520.1 DUF645 family protein -
ASZ88_RS18480 829558..830055 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
ASZ88_RS18485 830052..830324 - 273 WP_000246253.1 DUF1778 domain-containing protein -
ASZ88_RS18490 830555..831223 + 669 WP_000043871.1 hypothetical protein -
ASZ88_RS18495 831375..831662 + 288 WP_000426470.1 hypothetical protein -
ASZ88_RS18500 831803..832174 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
ASZ88_RS18505 832241..832666 + 426 WP_001882332.1 DUF1778 domain-containing protein -
ASZ88_RS18510 832663..833196 + 534 WP_000118351.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 688711..843534 154823
- inside Integron catB9 - 693791..843158 149367
flank IS/Tn - - 824372..825352 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(11-71)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10991.96 Da        Isoelectric Point: 10.4934

>T58340 WP_000221354.1 NZ_CP013312:828012-828299 [Vibrio cholerae]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Download         Length: 288 bp

>T58340 NZ_CP013312:828012-828299 [Vibrio cholerae]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9136.29 Da        Isoelectric Point: 4.0197

>AT58340 WP_001250179.1 NZ_CP013312:827771-828022 [Vibrio cholerae]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL

Download         Length: 252 bp

>AT58340 NZ_CP013312:827771-828022 [Vibrio cholerae]
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X1KZS6

Antitoxin

Source ID Structure
AlphaFold DB A0A0X1KZS8

References