Detailed information of TA system TA058366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 457171..458126
• Replicon: chromosome
• Accession: NZ_CP013314
• Organism: Vibrio cholerae strain E9120

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: ASZ79_RS16970
• Protein ID: WP_000118351.1
• Coordinates: 457593..458126 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ASZ79_RS16965
• Protein ID: WP_001882332.1
• Coordinates: 457171..457596 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ79_RS16915	452701..452952	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ79_RS16920	452942..453229	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ79_RS16925	453357..453812	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ79_RS16935	454134..454286	+	153	WP_001884520.1	DUF645 family protein	-
ASZ79_RS16940	454488..454985	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
ASZ79_RS16945	454982..455254	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
ASZ79_RS16950	455485..456153	+	669	WP_000043871.1	hypothetical protein	-
ASZ79_RS16955	456305..456592	+	288	WP_000426470.1	hypothetical protein	-
ASZ79_RS16960	456733..457104	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ79_RS16965	457171..457596	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
ASZ79_RS16970	457593..458126	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
ASZ79_RS16975	458328..458585	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ79_RS16980	458573..458875	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ79_RS16985	459109..460026	+	918	WP_000186333.1	alpha/beta hydrolase	-
ASZ79_RS16995	460183..460572	+	390	WP_001081302.1	hypothetical protein	-
ASZ79_RS17000	460708..460917	+	210	Protein_519	GNAT family N-acetyltransferase	-
ASZ79_RS17005	461036..461473	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
ASZ79_RS17010	461537..461755	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
ASZ79_RS17020	461930..462802	+	873	WP_000254716.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304700..468464	163764
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309780..468088	158308
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	461036..461473	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T58366 WP_000118351.1 NZ_CP013314:457593-458126 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T58366 NZ_CP013314:457593-458126 [Vibrio cholerae]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT58366 WP_001882332.1 NZ_CP013314:457171-457596 [Vibrio cholerae]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT58366 NZ_CP013314:457171-457596 [Vibrio cholerae]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94