Detailed information of TA system TA088997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 416307..417400
• Replicon: chromosome
• Accession: NZ_CP024868
• Organism: Vibrio cholerae strain A1552

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: VCA1552_RS16760
• Protein ID: WP_000351248.1
• Coordinates: 416999..417400 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: VCA1552_RS16755
• Protein ID: WP_001047169.1
• Coordinates: 416307..416999 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VCA1552_RS16690	411590..411967	+	378	WP_000411109.1	hypothetical protein	-
VCA1552_RS20035	412024..412138	+	115	Protein_434	acetyltransferase	-
VCA1552_RS16695	412087..412368	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
VCA1552_RS16710	412534..412647	+	114	WP_001889158.1	hypothetical protein	-
VCA1552_RS16715	412596..412823	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
VCA1552_RS16730	413164..413496	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCA1552_RS16735	413483..413797	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
VCA1552_RS16740	413934..414368	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
VCA1552_RS19910	414536..414691	+	156	WP_000751734.1	hypothetical protein	-
VCA1552_RS16745	414816..415796	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
VCA1552_RS16750	415864..416136	+	273	Protein_443	CatB-related O-acetyltransferase	-
VCA1552_RS16755	416307..416999	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
VCA1552_RS16760	416999..417400	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
VCA1552_RS16765	417370..417513	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
VCA1552_RS16770	417588..418001	+	414	WP_000049417.1	VOC family protein	-
VCA1552_RS16775	418215..418466	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VCA1552_RS16780	418456..418743	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCA1552_RS16785	418871..419326	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
VCA1552_RS16790	419648..419800	+	153	WP_001884520.1	DUF645 family protein	-
VCA1552_RS16800	420002..420499	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
VCA1552_RS16805	420496..420768	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
VCA1552_RS16810	420999..421667	+	669	WP_000043871.1	hypothetical protein	-
VCA1552_RS16815	421819..422106	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304717..433978	129261
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	309797..433602	123805
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	414816..415796	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T88997 WP_000351248.1 NZ_CP024868:416999-417400 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T88997 NZ_CP024868:416999-417400 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT88997 WP_001047169.1 NZ_CP024868:416307-416999 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT88997 NZ_CP024868:416307-416999 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34