Detailed information of TA system TA057661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 848289..849382
• Replicon: chromosome
• Accession: NZ_CP012997
• Organism: Vibrio cholerae strain 2012Env-9

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: NH62_RS03870
• Protein ID: WP_000351248.1
• Coordinates: 848289..848690 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: NH62_RS03875
• Protein ID: WP_001047169.1
• Coordinates: 848690..849382 (-)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NH62_RS03825	843313..844293	-	981	WP_000019368.1	IS5-like element ISVch5 family transposase	-
NH62_RS03830	844363..844689	-	327	Protein_746	hypothetical protein	-
NH62_RS03835	844920..845192	+	273	WP_000246253.1	DUF1778 domain-containing protein	-
NH62_RS03840	845189..845686	+	498	WP_000982260.1	GNAT family N-acetyltransferase	-
NH62_RS03845	845889..846041	-	153	WP_001884520.1	DUF645 family protein	-
NH62_RS03850	846363..846818	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
NH62_RS03855	846946..847233	-	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
NH62_RS03860	847223..847474	-	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
NH62_RS03865	847688..848101	-	414	WP_000049417.1	VOC family protein	-
NH62_RS19860	848176..848319	-	144	WP_071908341.1	DUF3265 domain-containing protein	-
NH62_RS03870	848289..848690	-	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
NH62_RS03875	848690..849382	-	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
NH62_RS03880	849519..849825	-	307	Protein_757	CatB-related O-acetyltransferase	-
NH62_RS03885	849893..850873	+	981	WP_000019368.1	IS5-like element ISVch5 family transposase	-
NH62_RS03890	850998..851198	-	201	WP_001198130.1	hypothetical protein	-
NH62_RS19865	851558..851731	-	174	WP_001882305.1	DUF3709 domain-containing protein	-
NH62_RS03895	852024..852575	-	552	WP_000821930.1	hypothetical protein	-
NH62_RS03900	852731..853588	-	858	WP_001279526.1	DUF2806 domain-containing protein	-
NH62_RS03905	853735..854349	-	615	WP_000789577.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	827256..947647	120391
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0.001	flank	IS/Tn	-	-	843313..844293	980
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0.001	flank	IS/Tn	-	-	849893..850873	980
	-	inside	Integron	-	-	828501..941266	112765

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T57661 WP_000351248.1 NZ_CP012997:c848690-848289 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T57661 NZ_CP012997:c848690-848289 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT57661 WP_001047169.1 NZ_CP012997:c849382-848690 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT57661 NZ_CP012997:c849382-848690 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34