Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 395694..396222 Replicon chromosome II
Accession NC_012580
Organism Vibrio cholerae M66-2

Toxin (Protein)


Gene name relE Uniprot ID A0A0X1KZS6
Locus tag VCM66_RS15340 Protein ID WP_000221354.1
Coordinates 395935..396222 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0X1KZS8
Locus tag VCM66_RS15335 Protein ID WP_001250179.1
Coordinates 395694..395945 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VCM66_RS15295 390962..391276 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
VCM66_RS15300 391413..391847 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
VCM66_RS19590 392015..392170 + 156 WP_000751734.1 hypothetical protein -
VCM66_RS15310 392295..393275 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
VCM66_RS15315 393343..393615 + 273 Protein_401 CatB-related O-acetyltransferase -
VCM66_RS15320 393786..394478 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
VCM66_RS15325 394478..394879 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
VCM66_RS19425 394849..394992 + 144 WP_071908341.1 DUF3265 domain-containing protein -
VCM66_RS15330 395067..395480 + 414 WP_000049417.1 VOC family protein -
VCM66_RS15335 395694..395945 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
VCM66_RS15340 395935..396222 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
VCM66_RS15345 396350..396805 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
VCM66_RS19005 397127..397279 + 153 WP_001884520.1 DUF645 family protein -
VCM66_RS15350 397481..397978 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
VCM66_RS15355 397975..398247 - 273 WP_000246253.1 DUF1778 domain-containing protein -
VCM66_RS15360 398478..399146 + 669 WP_000043871.1 hypothetical protein -
VCM66_RS15365 399298..399585 + 288 WP_000426470.1 hypothetical protein -
VCM66_RS15370 399726..400097 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
VCM66_RS19595 400164..400589 + 426 WP_001882332.1 DUF1778 domain-containing protein -
VCM66_RS15380 400586..401119 + 534 WP_000118351.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 305869..409778 103909
- inside Integron - - 310949..409402 98453
flank IS/Tn - - 392295..393275 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(11-71)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10991.96 Da        Isoelectric Point: 10.4934

>T23715 WP_000221354.1 NC_012580:395935-396222 [Vibrio cholerae M66-2]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Download         Length: 288 bp

>T23715 NC_012580:395935-396222 [Vibrio cholerae M66-2]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9136.29 Da        Isoelectric Point: 4.0197

>AT23715 WP_001250179.1 NC_012580:395694-395945 [Vibrio cholerae M66-2]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL

Download         Length: 252 bp

>AT23715 NC_012580:395694-395945 [Vibrio cholerae M66-2]
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X1KZS6

Antitoxin

Source ID Structure
AlphaFold DB A0A0X1KZS8

References