Detailed information of TA system TA058315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 575845..576478
• Replicon: chromosome
• Accession: NZ_CP013310
• Organism: Vibrio cholerae strain E1162

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: ASZ87_RS17620
• Protein ID: WP_000843587.1
• Coordinates: 575845..576177 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ87_RS17625
• Protein ID: WP_000071008.1
• Coordinates: 576164..576478 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ87_RS21005	571286..571489	+	204	WP_001911745.1	hypothetical protein	-
ASZ87_RS20805	571770..571943	+	174	WP_001882305.1	DUF3709 domain-containing protein	-
ASZ87_RS17560	572303..572572	+	270	WP_001198131.1	hypothetical protein	-
ASZ87_RS21165	572890..573061	+	172	Protein_597	DUF645 family protein	-
ASZ87_RS17575	573270..573656	+	387	WP_000703163.1	VOC family protein	-
ASZ87_RS17580	573858..574100	+	243	WP_000107461.1	hypothetical protein	-
ASZ87_RS17585	574271..574648	+	378	WP_000411109.1	hypothetical protein	-
ASZ87_RS21130	574705..574819	+	115	Protein_601	acetyltransferase	-
ASZ87_RS17590	574768..575049	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
ASZ87_RS17605	575215..575328	+	114	WP_001889158.1	hypothetical protein	-
ASZ87_RS17610	575277..575504	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
ASZ87_RS17620	575845..576177	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ87_RS17625	576164..576478	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
ASZ87_RS17630	576615..577049	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
ASZ87_RS20915	577217..577372	+	156	WP_000751734.1	hypothetical protein	-
ASZ87_RS17645	577497..578477	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
ASZ87_RS17650	578545..578851	+	307	Protein_610	CatB-related O-acetyltransferase	-
ASZ87_RS17655	578988..579680	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
ASZ87_RS17660	579680..580081	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
ASZ87_RS20820	580051..580194	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
ASZ87_RS17670	580269..580682	+	414	WP_000049417.1	VOC family protein	-
ASZ87_RS17675	580896..581147	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ87_RS17680	581137..581424	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	435534..596659	161125
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	440614..596283	155669
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	577497..578477	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T58315 WP_000843587.1 NZ_CP013310:575845-576177 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T58315 NZ_CP013310:575845-576177 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT58315 WP_000071008.1 NZ_CP013310:576164-576478 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT58315 NZ_CP013310:576164-576478 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA