Detailed information of TA system TA058389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 219242..219801
• Replicon: chromosome
• Accession: NZ_CP013316
• Organism: Vibrio cholerae strain M2140

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q9K2M8
• Locus tag: ASZ83_RS15620
• Protein ID: WP_000578476.1
• Coordinates: 219242..219520 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9L991
• Locus tag: ASZ83_RS15625
• Protein ID: WP_000381183.1
• Coordinates: 219517..219801 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ83_RS15570	214542..214979	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
ASZ83_RS15575	215043..215261	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
ASZ83_RS15585	215436..216308	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
ASZ83_RS15590	216458..217057	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
ASZ83_RS20230	217114..217218	+	105	WP_099607150.1	acetyltransferase	-
ASZ83_RS15595	217249..217518	+	270	WP_001114075.1	DUF4160 domain-containing protein	-
ASZ83_RS15600	217512..218033	+	522	WP_000921691.1	DUF2442 domain-containing protein	-
ASZ83_RS20235	218332..218457	+	126	WP_001905700.1	DUF645 family protein	-
ASZ83_RS15615	218708..219103	+	396	WP_001000867.1	hypothetical protein	-
ASZ83_RS15620	219242..219520	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
ASZ83_RS15625	219517..219801	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ASZ83_RS15630	219995..220510	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
ASZ83_RS15635	220694..221107	+	414	WP_000049420.1	VOC family protein	-
ASZ83_RS15650	221662..222839	+	1178	WP_086010879.1	IS3-like element ISVch4 family transposase	-
ASZ83_RS15655	222832..224211	-	1380	WP_000149603.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	87981..224211	136230
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.008	inside	Integron	-	-	93061..221594	128533
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0.002	flank	IS/Tn	-	-	214542..214979	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10819.59 Da
• Isoelectric Point: 5.1651

>T58389 WP_000578476.1 NZ_CP013316:c219520-219242 [Vibrio cholerae]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Nucleotide

• Length: 279 bp

>T58389 NZ_CP013316:c219520-219242 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10901.25 Da
• Isoelectric Point: 8.0775

>AT58389 WP_000381183.1 NZ_CP013316:c219801-219517 [Vibrio cholerae]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Nucleotide

• Length: 285 bp

>AT58389 NZ_CP013316:c219801-219517 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Structures

Toxin

• AlphaFold DB: Q9K2M8

Antitoxin

• AlphaFold DB: A0A067BL33