Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 587714..588242 Replicon chromosome
Accession NZ_CP046743
Organism Vibrio cholerae strain 3569-08

Toxin (Protein)


Gene name relE Uniprot ID A0A0X1KZS6
Locus tag GPW72_RS02700 Protein ID WP_000221354.1
Coordinates 587714..588001 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0X1KZS8
Locus tag GPW72_RS02705 Protein ID WP_001250179.1
Coordinates 587991..588242 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPW72_RS02650 582817..583350 - 534 WP_000118351.1 GNAT family N-acetyltransferase -
GPW72_RS19170 583347..583772 - 426 WP_001882332.1 DUF1778 domain-containing protein -
GPW72_RS02660 583839..584210 - 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
GPW72_RS02665 584351..584638 - 288 WP_000426470.1 hypothetical protein -
GPW72_RS02670 584790..585458 - 669 WP_000043871.1 hypothetical protein -
GPW72_RS02675 585689..585961 + 273 WP_000246253.1 DUF1778 domain-containing protein -
GPW72_RS02680 585958..586455 + 498 WP_000982260.1 GNAT family N-acetyltransferase -
GPW72_RS02690 586657..586809 - 153 WP_001884520.1 DUF645 family protein -
GPW72_RS02695 587131..587586 - 456 WP_001245327.1 GNAT family N-acetyltransferase -
GPW72_RS02700 587714..588001 - 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GPW72_RS02705 587991..588242 - 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GPW72_RS02710 588456..588869 - 414 WP_000049417.1 VOC family protein -
GPW72_RS02715 588944..589087 - 144 WP_071908341.1 DUF3265 domain-containing protein -
GPW72_RS02720 589057..589458 - 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
GPW72_RS02725 589458..590150 - 693 WP_001047169.1 GNAT family N-acetyltransferase -
GPW72_RS02730 590287..590593 - 307 Protein_526 CatB-related O-acetyltransferase -
GPW72_RS02735 590661..591641 + 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
GPW72_RS02740 591766..591921 - 156 WP_000751734.1 hypothetical protein -
GPW72_RS02745 592089..592523 - 435 WP_000256036.1 GNAT family N-acetyltransferase -
GPW72_RS02750 592660..592974 - 315 WP_000071008.1 DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 575627..724145 148518
- inside Integron - - 575627..717201 141574
flank IS/Tn - - 590661..591641 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(11-71)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10991.96 Da        Isoelectric Point: 10.4934

>T144872 WP_000221354.1 NZ_CP046743:c588001-587714 [Vibrio cholerae]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Download         Length: 288 bp

>T144872 NZ_CP062244:c2082946-2082692 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9136.29 Da        Isoelectric Point: 4.0197

>AT144872 WP_001250179.1 NZ_CP046743:c588242-587991 [Vibrio cholerae]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL

Download         Length: 252 bp

>AT144872 NZ_CP062244:c2083194-2082943 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X1KZS6

Antitoxin

Source ID Structure
AlphaFold DB A0A0X1KZS8

References