Detailed information of TA system TA058319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 585366..586321
• Replicon: chromosome
• Accession: NZ_CP013310
• Organism: Vibrio cholerae strain E1162

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: ASZ87_RS17730
• Protein ID: WP_000118351.1
• Coordinates: 585788..586321 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ASZ87_RS17725
• Protein ID: WP_001882332.1
• Coordinates: 585366..585791 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ87_RS17675	580896..581147	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ87_RS17680	581137..581424	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ87_RS17685	581552..582007	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ87_RS17695	582329..582481	+	153	WP_001884520.1	DUF645 family protein	-
ASZ87_RS17700	582683..583180	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
ASZ87_RS17705	583177..583449	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
ASZ87_RS17710	583680..584348	+	669	WP_000043871.1	hypothetical protein	-
ASZ87_RS17715	584500..584787	+	288	WP_000426470.1	hypothetical protein	-
ASZ87_RS17720	584928..585299	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ87_RS17725	585366..585791	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
ASZ87_RS17730	585788..586321	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
ASZ87_RS17735	586523..586780	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ87_RS17740	586768..587070	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ87_RS17745	587304..588221	+	918	WP_000186333.1	alpha/beta hydrolase	-
ASZ87_RS17755	588378..588767	+	390	WP_001081302.1	hypothetical protein	-
ASZ87_RS17760	588903..589112	+	210	Protein_630	GNAT family N-acetyltransferase	-
ASZ87_RS17765	589231..589668	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
ASZ87_RS17770	589732..589950	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
ASZ87_RS17780	590125..590997	+	873	WP_000254716.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	435534..596659	161125
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	440614..596283	155669
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	589231..589668	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T58319 WP_000118351.1 NZ_CP013310:585788-586321 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T58319 NZ_CP013310:585788-586321 [Vibrio cholerae]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT58319 WP_001882332.1 NZ_CP013310:585366-585791 [Vibrio cholerae]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT58319 NZ_CP013310:585366-585791 [Vibrio cholerae]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94