Detailed information of TA system TA125044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 957028..957661
• Replicon: chromosome
• Accession: NZ_CP040173
• Organism: Vibrio cholerae strain VC1374

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: FDZ90_RS19180
• Protein ID: WP_000843587.1
• Coordinates: 957028..957360 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FDZ90_RS19185
• Protein ID: WP_000071008.1
• Coordinates: 957347..957661 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDZ90_RS19120	952471..952674	+	204	WP_001911745.1	hypothetical protein	-
FDZ90_RS19125	952955..953128	+	174	WP_001882305.1	DUF3709 domain-containing protein	-
FDZ90_RS19130	953488..953757	+	270	WP_001198131.1	hypothetical protein	-
FDZ90_RS19135	954068..954154	+	87	WP_134820423.1	DUF645 family protein	-
FDZ90_RS19140	954154..954246	+	93	Protein_881	DUF645 family protein	-
FDZ90_RS19145	954455..954841	+	387	WP_000703163.1	VOC family protein	-
FDZ90_RS19150	955043..955285	+	243	WP_000107461.1	hypothetical protein	-
FDZ90_RS19155	955455..955832	+	378	WP_000411109.1	hypothetical protein	-
FDZ90_RS19160	955889..956003	+	115	Protein_885	acetyltransferase	-
FDZ90_RS19165	955952..956233	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
FDZ90_RS19170	956398..956511	+	114	WP_001889158.1	hypothetical protein	-
FDZ90_RS19175	956460..956687	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
FDZ90_RS19180	957028..957360	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FDZ90_RS19185	957347..957661	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
FDZ90_RS19190	957798..958232	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
FDZ90_RS19195	958400..958555	+	156	WP_000751734.1	hypothetical protein	-
FDZ90_RS19200	958680..959659	-	980	Protein_893	IS5-like element ISVch5 family transposase	-
FDZ90_RS19205	959727..959999	+	273	Protein_894	CatB-related O-acetyltransferase	-
FDZ90_RS19210	960170..960862	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
FDZ90_RS19215	960862..961263	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
FDZ90_RS19220	961233..961376	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
FDZ90_RS19225	961451..961864	+	414	WP_000049417.1	VOC family protein	-
FDZ90_RS19230	962078..962329	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FDZ90_RS19235	962319..962606	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	882589..977840	95251
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	catB9	-	887669..977464	89795
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0.009	flank	IS/Tn	-	-	958940..959659	719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T125044 WP_000843587.1 NZ_CP040173:957028-957360 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T125044 NZ_CP040173:957028-957360 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT125044 WP_000071008.1 NZ_CP040173:957347-957661 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT125044 NZ_CP040173:957347-957661 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA