23714

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-GNAT
Location 393786..394879 Replicon chromosome II
Accession NC_012580
Organism Vibrio cholerae M66-2

Toxin (Protein)


Gene name doc Uniprot ID Q9KMA2
Locus tag VCM66_RS15325 Protein ID WP_000351248.1
Coordinates 394478..394879 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID -
Locus tag VCM66_RS15320 Protein ID WP_001047169.1
Coordinates 393786..394478 (+) Length 231 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VCM66_RS15280 389069..389446 + 378 WP_000411109.1 hypothetical protein -
VCM66_RS19585 389503..389617 + 115 Protein_392 acetyltransferase -
VCM66_RS18970 389566..389847 + 282 WP_001894453.1 DUF1289 domain-containing protein -
VCM66_RS18985 390013..390126 + 114 WP_001889158.1 hypothetical protein -
VCM66_RS18990 390075..390302 + 228 WP_001894457.1 DUF1289 domain-containing protein -
VCM66_RS15290 390643..390975 + 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin -
VCM66_RS15295 390962..391276 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
VCM66_RS15300 391413..391847 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
VCM66_RS19590 392015..392170 + 156 WP_000751734.1 hypothetical protein -
VCM66_RS15310 392295..393275 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
VCM66_RS15315 393343..393615 + 273 Protein_401 CatB-related O-acetyltransferase -
VCM66_RS15320 393786..394478 + 693 WP_001047169.1 GNAT family N-acetyltransferase Antitoxin
VCM66_RS15325 394478..394879 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
VCM66_RS19425 394849..394992 + 144 WP_071908341.1 DUF3265 domain-containing protein -
VCM66_RS15330 395067..395480 + 414 WP_000049417.1 VOC family protein -
VCM66_RS15335 395694..395945 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
VCM66_RS15340 395935..396222 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
VCM66_RS15345 396350..396805 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
VCM66_RS19005 397127..397279 + 153 WP_001884520.1 DUF645 family protein -
VCM66_RS15350 397481..397978 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
VCM66_RS15355 397975..398247 - 273 WP_000246253.1 DUF1778 domain-containing protein -
VCM66_RS15360 398478..399146 + 669 WP_000043871.1 hypothetical protein -
VCM66_RS15365 399298..399585 + 288 WP_000426470.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 305869..409778 103909
- inside Integron - - 310949..409402 98453
flank IS/Tn - - 392295..393275 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-132)

Antitoxin

GNAT domain

(36-153)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14608.71 Da        Isoelectric Point: 4.0128

>T23714 WP_000351248.1 NC_012580:394478-394879 [Vibrio cholerae M66-2]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Download         Length: 402 bp

>T23714 NC_012580:394478-394879 [Vibrio cholerae M66-2]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin


Download         Length: 231 a.a.        Molecular weight: 26352.73 Da        Isoelectric Point: 8.5633

>AT23714 WP_001047169.1 NC_012580:393786-394478 [Vibrio cholerae M66-2]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Download         Length: 693 bp

>AT23714 NC_012580:393786-394478 [Vibrio cholerae M66-2]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0PN34

Antitoxin

Source ID Structure

References