Detailed information of TA system TA023716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 397481..398247
• Replicon: chromosome II
• Accession: NC_012580
• Organism: Vibrio cholerae M66-2

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: VCM66_RS15350
• Protein ID: WP_000982260.1
• Coordinates: 397481..397978 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2J6
• Locus tag: VCM66_RS15355
• Protein ID: WP_000246253.1
• Coordinates: 397975..398247 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VCM66_RS15315	393343..393615	+	273	Protein_401	CatB-related O-acetyltransferase	-
VCM66_RS15320	393786..394478	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
VCM66_RS15325	394478..394879	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
VCM66_RS19425	394849..394992	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
VCM66_RS15330	395067..395480	+	414	WP_000049417.1	VOC family protein	-
VCM66_RS15335	395694..395945	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VCM66_RS15340	395935..396222	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCM66_RS15345	396350..396805	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
VCM66_RS19005	397127..397279	+	153	WP_001884520.1	DUF645 family protein	-
VCM66_RS15350	397481..397978	-	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
VCM66_RS15355	397975..398247	-	273	WP_000246253.1	DUF1778 domain-containing protein	Antitoxin
VCM66_RS15360	398478..399146	+	669	WP_000043871.1	hypothetical protein	-
VCM66_RS15365	399298..399585	+	288	WP_000426470.1	hypothetical protein	-
VCM66_RS15370	399726..400097	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
VCM66_RS19595	400164..400589	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
VCM66_RS15380	400586..401119	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
VCM66_RS15385	401321..401578	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VCM66_RS15390	401566..401868	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCM66_RS15395	402022..402231	+	210	Protein_419	GNAT family N-acetyltransferase	-
VCM66_RS15400	402350..402787	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
VCM66_RS15405	402851..403069	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	305869..409778	103909
	-	inside	Integron	-	-	310949..409402	98453
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	392295..393275	980
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	402350..402787	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T23716 WP_000982260.1 NC_012580:c397978-397481 [Vibrio cholerae M66-2]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T23716 NC_012580:c397978-397481 [Vibrio cholerae M66-2]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9683.22 Da
• Isoelectric Point: 6.2988

>AT23716 WP_000246253.1 NC_012580:c398247-397975 [Vibrio cholerae M66-2]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT23716 NC_012580:c398247-397975 [Vibrio cholerae M66-2]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1

Antitoxin

• AlphaFold DB: A0A0F2IC79