Detailed information of TA system TA058410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/-
• Location: 492112..492896
• Replicon: chromosome
• Accession: NZ_CP013318
• Organism: Vibrio cholerae strain NCTC5395

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A086SLD6
• Locus tag: ASZ85_RS17185
• Protein ID: WP_001114075.1
• Coordinates: 492112..492381 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: Q9KM84
• Locus tag: ASZ85_RS17190
• Protein ID: WP_000921691.1
• Coordinates: 492375..492896 (+)
• Length: 174 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ85_RS17140	487478..488395	+	918	WP_000186333.1	alpha/beta hydrolase	-
ASZ85_RS17150	488552..488941	+	390	WP_001081302.1	hypothetical protein	-
ASZ85_RS17155	489077..489286	+	210	Protein_549	GNAT family N-acetyltransferase	-
ASZ85_RS17160	489405..489842	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
ASZ85_RS17165	489906..490124	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
ASZ85_RS17175	490299..491171	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
ASZ85_RS17180	491321..491920	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
ASZ85_RS21230	491977..492081	+	105	WP_099607150.1	acetyltransferase	-
ASZ85_RS17185	492112..492381	+	270	WP_001114075.1	DUF4160 domain-containing protein	Toxin
ASZ85_RS17190	492375..492896	+	522	WP_000921691.1	DUF2442 domain-containing protein	Antitoxin
ASZ85_RS21235	493195..493320	+	126	WP_001905700.1	DUF645 family protein	-
ASZ85_RS17205	493571..493966	+	396	WP_001000867.1	hypothetical protein	-
ASZ85_RS17210	494105..494383	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
ASZ85_RS17215	494380..494664	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
ASZ85_RS17220	494858..495373	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
ASZ85_RS17225	495557..495970	+	414	WP_000049420.1	VOC family protein	-
ASZ85_RS17240	496525..497702	+	1178	WP_086010879.1	IS3-like element ISVch4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	338285..496833	158548
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.009	inside	Integron	-	-	343365..496457	153092
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	489405..489842	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Protein of unknown function DUF2442 (4-59)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.94 Da
• Isoelectric Point: 6.6303

>T58410 WP_001114075.1 NZ_CP013318:492112-492381 [Vibrio cholerae]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Nucleotide

• Length: 270 bp

>T58410 NZ_CP013318:492112-492381 [Vibrio cholerae]
ATGCCAGAGATTGATGCCCTATTAGGTCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTACCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AACGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin

Protein

• Length: 174 a.a.
• Molecular weight: 19409.01 Da
• Isoelectric Point: 6.7342

>AT58410 WP_000921691.1 NZ_CP013318:492375-492896 [Vibrio cholerae]
MLKVIDVDFVSDHTLELTFNDGYQGYADLSVYFKKAPFSEIKDFKRFSLTRDGSLNWDGNELTAATLRDITKGSQKSVEL
SFNVQEMEAVIKQASWESMMEGRPDILQAAIRSYVEQFGHGQVIAKAGIKSRTSAYRSLKPETTPNFGTLVQLGHAVIEL
AKDRTVANKEPRI

Nucleotide

• Length: 522 bp

>AT58410 NZ_CP013318:492375-492896 [Vibrio cholerae]
ATGTTGAAAGTCATAGATGTTGATTTTGTCTCAGACCATACATTAGAACTGACGTTCAATGATGGTTACCAAGGCTATGC
CGACTTGTCGGTATACTTTAAAAAAGCACCGTTTTCAGAAATTAAGGACTTTAAACGCTTTTCCTTGACTCGTGATGGCT
CGTTAAATTGGGATGGTAATGAGTTAACGGCCGCGACACTCCGTGACATAACCAAGGGCTCACAAAAGTCGGTGGAGTTA
AGTTTTAATGTTCAAGAAATGGAAGCCGTCATCAAACAGGCCTCATGGGAATCGATGATGGAGGGGCGTCCCGACATCTT
GCAAGCTGCTATTCGTTCTTACGTAGAGCAGTTTGGTCATGGTCAAGTTATTGCAAAGGCGGGTATAAAAAGTCGTACGA
GTGCTTATCGCTCCTTGAAGCCAGAAACAACGCCAAACTTTGGCACACTAGTTCAGTTAGGGCACGCCGTCATCGAACTA
GCGAAAGATAGAACAGTGGCAAACAAGGAACCACGCATATAA

Structures

Toxin

• AlphaFold DB: A0A086SLD6

Antitoxin

• AlphaFold DB: Q9KM84