Detailed information of TA system TA058424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 415424..416057
• Replicon: chromosome
• Accession: NZ_CP013320
• Organism: Vibrio cholerae strain NCTC9420

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: ASZ86_RS16635
• Protein ID: WP_000843587.1
• Coordinates: 415424..415756 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ86_RS16640
• Protein ID: WP_000071008.1
• Coordinates: 415743..416057 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ86_RS20720	410865..411068	+	204	WP_001911745.1	hypothetical protein	-
ASZ86_RS20530	411349..411522	+	174	WP_001882305.1	DUF3709 domain-containing protein	-
ASZ86_RS16575	411882..412151	+	270	WP_001198131.1	hypothetical protein	-
ASZ86_RS20835	412469..412640	+	172	Protein_434	DUF645 family protein	-
ASZ86_RS16590	412849..413235	+	387	WP_000703163.1	VOC family protein	-
ASZ86_RS16595	413437..413679	+	243	WP_000107461.1	hypothetical protein	-
ASZ86_RS16600	413850..414227	+	378	WP_071919850.1	hypothetical protein	-
ASZ86_RS20810	414284..414398	+	115	Protein_438	acetyltransferase	-
ASZ86_RS16605	414347..414628	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
ASZ86_RS16620	414794..414907	+	114	WP_001889158.1	hypothetical protein	-
ASZ86_RS16625	414856..415083	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
ASZ86_RS16635	415424..415756	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ86_RS16640	415743..416057	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
ASZ86_RS16645	416194..416628	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
ASZ86_RS20650	416796..416951	+	156	WP_000751734.1	hypothetical protein	-
ASZ86_RS16660	417076..418056	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
ASZ86_RS16665	418124..418430	+	307	Protein_447	CatB-related O-acetyltransferase	-
ASZ86_RS16670	418567..419259	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
ASZ86_RS16675	419259..419660	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
ASZ86_RS20545	419630..419773	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
ASZ86_RS16685	419848..420261	+	414	WP_000049417.1	VOC family protein	-
ASZ86_RS16690	420475..420726	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ86_RS16695	420716..421003	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304702..436238	131536
	-	inside	Integron	-	-	309782..435862	126080
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	417076..418056	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T58424 WP_000843587.1 NZ_CP013320:415424-415756 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T58424 NZ_CP013320:415424-415756 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT58424 WP_000071008.1 NZ_CP013320:415743-416057 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT58424 NZ_CP013320:415743-416057 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA