Detailed information of TA system TA000303

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Xre-RelE
• Location: 1373329..1373951
• Replicon: chromosome
• Accession: NC_002947
• Organism: Pseudomonas putida KT2440

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q88NK8
• Locus tag: PP_1199
• Protein ID: 26987934
• Coordinates: 1373618..1373932 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q88NK9
• Locus tag: PP_1198
• Protein ID: 26987933
• Coordinates: 1373331..1373618 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PP_RS06180 (PP_1194)	1368363..1369208	+	846	WP_003254799.1	hypothetical protein	-
PP_RS06185 (PP_1195)	1369205..1370923	+	1719	WP_010952348.1	hypothetical protein	-
PP_RS06190 (PP_1196)	1370978..1371727	+	750	WP_049587988.1	hypothetical protein	-
PP_RS06195 (PP_1197)	1371799..1373130	-	1332	WP_004576169.1	30S ribosomal protein S12 methylthiotransferase RimO	-
PP_RS06200 (PP_1198)	1373331..1373618	-	288	WP_049587986.1	helix-turn-helix domain-containing protein	Antitoxin
PP_RS06205 (PP_1199)	1373618..1373932	-	315	WP_049587984.1	hypothetical protein	Toxin
PP_RS06210 (PP_1200)	1374381..1376291	+	1911	WP_010952353.1	potassium transporter Kup	-
PP_RS06215 (PP_1201)	1376340..1377632	-	1293	WP_010952354.1	virulence factor family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-88)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11784.61 Da
• Isoelectric Point: 9.9097

>T303 26987934 NC_002947:c1373932-1373618 [Pseudomonas putida KT2440]
MIFIETPVFTSDLKEHLDDEEYRALQAYLAEHPEAGSLLEETGGLRKIRWAAKGKGKSGGVRVIYYHVTAAHQIRMILIY
RKGIVDTLTSSQKAQLRALNKGWK

Nucleotide

• Length: 315 bp

>T303 NC_002947:c1373932-1373618 [Pseudomonas putida KT2440]
ATGATCTTCATCGAGACCCCAGTATTCACCTCCGATCTCAAGGAGCACCTGGATGATGAAGAATACCGGGCTTTGCAGGC
CTATCTGGCCGAGCATCCCGAAGCCGGTTCTCTCCTGGAGGAAACCGGCGGCCTCAGAAAGATCCGCTGGGCAGCCAAGG
GCAAGGGCAAGAGTGGGGGTGTACGAGTGATCTACTACCACGTCACTGCAGCCCACCAGATCCGTATGATTCTGATCTAT
CGCAAGGGCATCGTGGATACCTTGACAAGCAGTCAGAAAGCGCAGCTTCGCGCACTAAACAAAGGGTGGAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10841.31 Da
• Isoelectric Point: 7.4063

>AT303 26987933 NC_002947:c1373618-1373331 [Pseudomonas putida KT2440]
MEKHLFDRLVESMTQMDEIDRGERQPSREFHVDALQVKKIRQATGLSQAAFAKRIDVAVGTLRNWEQGRREPEGPARALL
RAIHNDPEHVLAALS

Nucleotide

• Length: 288 bp

>AT303 NC_002947:c1373618-1373331 [Pseudomonas putida KT2440]
ATGGAAAAGCATCTCTTCGATCGTCTTGTGGAAAGCATGACTCAGATGGATGAGATCGATCGCGGTGAGCGTCAGCCCTC
GCGTGAGTTCCACGTAGACGCACTTCAAGTCAAAAAGATTCGTCAAGCCACTGGCCTTTCCCAGGCTGCGTTTGCCAAAC
GTATCGATGTCGCAGTAGGAACTCTACGGAATTGGGAACAGGGCCGTCGCGAACCTGAAGGACCAGCTCGTGCCTTGCTT
CGAGCGATTCATAACGATCCCGAACATGTACTTGCGGCCTTGAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6088	Salmonella enterica subsp. enterica serovar Typhimurium str. LT2	56.311	100	0.563
T174	Vibrio cholerae O1 biovar El Tor str. N16961	42.718	99.038	0.423

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT6088	Salmonella enterica subsp. enterica serovar Typhimurium str. LT2	65.854	97.619	0.643

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: Q88NK8

Antitoxin

• AlphaFold DB: Q88NK9

References

(1) Sirli Rosendahl et al. (2020) Chromosomal toxin-antitoxin systems in Pseudomonas putida are rather selfish than beneficial. Scientific Reports 10(1):9230. [PubMed:32513960]


 experimental literature