Detailed information of TA system TA038642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 867799..868432
• Replicon: chromosome
• Accession: NZ_AP024550
• Organism: Vibrio cholerae strain IDH-00113

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: K6J78_RS17635
• Protein ID: WP_000843587.1
• Coordinates: 867799..868131 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J78_RS17640
• Protein ID: WP_000071008.1
• Coordinates: 868118..868432 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J78_RS17580	863240..863443	+	204	WP_001911745.1	hypothetical protein	-
K6J78_RS17585	863700..863897	+	198	WP_001894451.1	DUF3709 domain-containing protein	-
K6J78_RS17590	864257..864526	+	270	WP_001198131.1	hypothetical protein	-
K6J78_RS17595	864862..865015	+	154	Protein_821	DUF645 family protein	-
K6J78_RS17600	865224..865610	+	387	WP_000703163.1	VOC family protein	-
K6J78_RS17605	865812..866054	+	243	WP_000107463.1	hypothetical protein	-
K6J78_RS17610	866225..866602	+	378	WP_000411109.1	hypothetical protein	-
K6J78_RS17615	866659..866773	+	115	Protein_825	acetyltransferase	-
K6J78_RS17620	866782..867003	+	222	WP_032468461.1	DUF1289 domain-containing protein	-
K6J78_RS17625	867169..867282	+	114	WP_001889158.1	hypothetical protein	-
K6J78_RS17630	867231..867458	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
K6J78_RS17635	867799..868131	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J78_RS17640	868118..868432	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
K6J78_RS17645	868569..869003	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
K6J78_RS17650	869171..869326	+	156	WP_000751734.1	hypothetical protein	-
K6J78_RS17655	869451..870431	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
K6J78_RS17660	870499..870805	+	307	Protein_834	CatB-related O-acetyltransferase	-
K6J78_RS18805	870942..871340	+	399	Protein_835	GNAT family N-acetyltransferase	-
K6J78_RS18810	871464..871634	+	171	WP_001080654.1	hypothetical protein	-
K6J78_RS17670	871634..872035	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
K6J78_RS18815	872038..872148	+	111	WP_082798255.1	DUF3265 domain-containing protein	-
K6J78_RS17675	872223..872636	+	414	WP_000049417.1	VOC family protein	-
K6J78_RS17680	872850..873101	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
K6J78_RS17685	873091..873378	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	782725..888585	105860
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	869451..870431	980
	-	inside	Integron	-	-	835106..888236	53130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T38642 WP_000843587.1 NZ_AP024550:867799-868131 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T38642 NZ_AP024550:867799-868131 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT38642 WP_000071008.1 NZ_AP024550:868118-868432 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT38642 NZ_AP024550:868118-868432 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA