Detailed information of TA system TA058249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 410003..411096
• Replicon: chromosome
• Accession: NZ_CP013304
• Organism: Vibrio cholerae strain CRC711

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: ASZ82_RS16560
• Protein ID: WP_000351248.1
• Coordinates: 410695..411096 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: ASZ82_RS16555
• Protein ID: WP_001047169.1
• Coordinates: 410003..410695 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ82_RS16490	405286..405663	+	378	WP_000411109.1	hypothetical protein	-
ASZ82_RS20480	405720..405834	+	115	Protein_420	acetyltransferase	-
ASZ82_RS16495	405783..406064	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
ASZ82_RS16510	406230..406343	+	114	WP_001889158.1	hypothetical protein	-
ASZ82_RS16515	406292..406519	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
ASZ82_RS16525	406860..407192	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ82_RS16530	407179..407493	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
ASZ82_RS16535	407630..408064	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
ASZ82_RS20270	408232..408387	+	156	WP_000751734.1	hypothetical protein	-
ASZ82_RS16545	408512..409492	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
ASZ82_RS16550	409560..409866	+	307	Protein_429	CatB-related O-acetyltransferase	-
ASZ82_RS16555	410003..410695	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
ASZ82_RS16560	410695..411096	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ASZ82_RS20175	411066..411209	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
ASZ82_RS16570	411284..411697	+	414	WP_000049417.1	VOC family protein	-
ASZ82_RS16575	411911..412162	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ82_RS16580	412152..412439	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ82_RS16585	412567..413022	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ82_RS16595	413344..413496	+	153	WP_001884520.1	DUF645 family protein	-
ASZ82_RS16600	413698..414195	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
ASZ82_RS16605	414192..414464	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
ASZ82_RS16610	414695..415363	+	669	WP_000043871.1	hypothetical protein	-
ASZ82_RS16615	415515..415802	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304736..427674	122938
	-	inside	Integron	-	-	309816..427298	117482
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	408512..409492	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T58249 WP_000351248.1 NZ_CP013304:410695-411096 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T58249 NZ_CP013304:410695-411096 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT58249 WP_001047169.1 NZ_CP013304:410003-410695 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT58249 NZ_CP013304:410003-410695 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34