Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-YafN |
| Location | 72350..72878 | Replicon | chromosome |
| Accession | NZ_AP024556 | ||
| Organism | Vibrio cholerae strain IDH-03506 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | A0A0X1KZS6 |
| Locus tag | K6J79_RS14425 | Protein ID | WP_000221354.1 |
| Coordinates | 72591..72878 (+) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | A0A0X1KZS8 |
| Locus tag | K6J79_RS14420 | Protein ID | WP_001250179.1 |
| Coordinates | 72350..72601 (+) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K6J79_RS14380 | 67618..67932 | + | 315 | WP_000071008.1 | DNA-binding transcriptional regulator | - |
| K6J79_RS14385 | 68069..68503 | + | 435 | WP_000256036.1 | GNAT family N-acetyltransferase | - |
| K6J79_RS14390 | 68671..68826 | + | 156 | WP_000751734.1 | hypothetical protein | - |
| K6J79_RS14395 | 68951..69931 | - | 981 | WP_000019370.1 | IS5-like element ISVch5 family transposase | - |
| K6J79_RS14400 | 69999..70305 | + | 307 | Protein_143 | CatB-related O-acetyltransferase | - |
| K6J79_RS19000 | 70442..70840 | + | 399 | Protein_144 | GNAT family N-acetyltransferase | - |
| K6J79_RS19005 | 70964..71134 | + | 171 | WP_001080654.1 | hypothetical protein | - |
| K6J79_RS14410 | 71134..71535 | + | 402 | WP_000351248.1 | type II toxin-antitoxin system death-on-curing family toxin | - |
| K6J79_RS19010 | 71538..71648 | + | 111 | WP_082798255.1 | DUF3265 domain-containing protein | - |
| K6J79_RS14415 | 71723..72136 | + | 414 | WP_000049417.1 | VOC family protein | - |
| K6J79_RS14420 | 72350..72601 | + | 252 | WP_001250179.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| K6J79_RS14425 | 72591..72878 | + | 288 | WP_000221354.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| K6J79_RS14430 | 73006..73461 | + | 456 | WP_001245327.1 | GNAT family N-acetyltransferase | - |
| K6J79_RS19015 | 73518..73640 | + | 123 | Protein_152 | acetyltransferase | - |
| K6J79_RS14435 | 73811..73935 | + | 125 | Protein_153 | DUF645 family protein | - |
| K6J79_RS19020 | 74052..74120 | + | 69 | Protein_154 | acetyltransferase | - |
| K6J79_RS14440 | 74137..74633 | - | 497 | Protein_155 | GNAT family N-acetyltransferase | - |
| K6J79_RS14445 | 74630..74902 | - | 273 | WP_000246253.1 | DUF1778 domain-containing protein | - |
| K6J79_RS19025 | 75054..75116 | + | 63 | Protein_157 | acetyltransferase | - |
| K6J79_RS14450 | 75133..75801 | + | 669 | WP_000043871.1 | hypothetical protein | - |
| K6J79_RS14455 | 75953..76240 | + | 288 | WP_000426470.1 | hypothetical protein | - |
| K6J79_RS14460 | 76381..76752 | + | 372 | WP_001164080.1 | MazG nucleotide pyrophosphohydrolase domain-containing protein | - |
| K6J79_RS19030 | 76975..77244 | + | 270 | WP_000179600.1 | DUF1778 domain-containing protein | - |
| K6J79_RS14470 | 77241..77774 | + | 534 | WP_000118351.1 | GNAT family N-acetyltransferase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integron | - | cqsA / ugd / htpB / hlyA / hcp-2 / vgrG-2 / vipA/mglA / vipB/mglB / VCA0109 / vasA / vasB / vasC / vasD / vasE / vasF / clpB/vasG / vasH / vasI / vasJ / icmF/vasK / vasL / vgrG-3 / hlyA | 3..1061958 | 1061955 | |
| flank | IS/Tn | - | - | 68951..69931 | 980 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 10991.96 Da Isoelectric Point: 10.4934
>T38697 WP_000221354.1 NZ_AP024556:72591-72878 [Vibrio cholerae]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG
Download Length: 288 bp
>T38697 NZ_AP024556:72591-72878 [Vibrio cholerae]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG
Antitoxin
Download Length: 84 a.a. Molecular weight: 9136.29 Da Isoelectric Point: 4.0197
>AT38697 WP_001250179.1 NZ_AP024556:72350-72601 [Vibrio cholerae]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL
Download Length: 252 bp
>AT38697 NZ_AP024556:72350-72601 [Vibrio cholerae]
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0X1KZS6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0X1KZS8 |