Detailed information of TA system TA025717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 122328..123094
• Replicon: chromosome
• Accession: NC_016446
• Organism: Vibrio cholerae O1 str. 2010EL-1786

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: HJ37_RS14820
• Protein ID: WP_000982260.1
• Coordinates: 122328..122825 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2J6
• Locus tag: HJ37_RS14825
• Protein ID: WP_000246253.1
• Coordinates: 122822..123094 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ37_RS14780	118190..118462	+	273	Protein_182	CatB-related O-acetyltransferase	-
HJ37_RS14785	118633..119325	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
HJ37_RS14790	119325..119726	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
HJ37_RS20025	119696..119839	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
HJ37_RS14795	119914..120327	+	414	WP_000049417.1	VOC family protein	-
HJ37_RS14800	120541..120792	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HJ37_RS14805	120782..121069	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HJ37_RS14810	121197..121652	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
HJ37_RS14815	121974..122126	+	153	WP_001884520.1	DUF645 family protein	-
HJ37_RS14820	122328..122825	-	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
HJ37_RS14825	122822..123094	-	273	WP_000246253.1	DUF1778 domain-containing protein	Antitoxin
HJ37_RS14830	123325..123993	+	669	WP_000043871.1	hypothetical protein	-
HJ37_RS14835	124145..124432	+	288	WP_000426470.1	hypothetical protein	-
HJ37_RS14840	124573..124944	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
HJ37_RS20245	125011..125436	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
HJ37_RS14850	125433..125966	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
HJ37_RS14855	126168..126425	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HJ37_RS14860	126413..126715	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HJ37_RS14865	126949..127866	+	918	WP_000186333.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31115..136304	105189
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	catB9	-	36195..135928	99733
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	117142..118122	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T25717 WP_000982260.1 NC_016446:c122825-122328 [Vibrio cholerae O1 str. 2010EL-1786]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T25717 NC_016446:c122825-122328 [Vibrio cholerae O1 str. 2010EL-1786]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9683.22 Da
• Isoelectric Point: 6.2988

>AT25717 WP_000246253.1 NC_016446:c123094-122822 [Vibrio cholerae O1 str. 2010EL-1786]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT25717 NC_016446:c123094-122822 [Vibrio cholerae O1 str. 2010EL-1786]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1

Antitoxin

• AlphaFold DB: A0A0F2IC79