Detailed information of TA system TA125047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 963865..964631
• Replicon: chromosome
• Accession: NZ_CP040173
• Organism: Vibrio cholerae strain VC1374

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: FDZ90_RS19250
• Protein ID: WP_000982260.1
• Coordinates: 963865..964362 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2J6
• Locus tag: FDZ90_RS19255
• Protein ID: WP_000246253.1
• Coordinates: 964359..964631 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDZ90_RS19205	959727..959999	+	273	Protein_894	CatB-related O-acetyltransferase	-
FDZ90_RS19210	960170..960862	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
FDZ90_RS19215	960862..961263	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
FDZ90_RS19220	961233..961376	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
FDZ90_RS19225	961451..961864	+	414	WP_000049417.1	VOC family protein	-
FDZ90_RS19230	962078..962329	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FDZ90_RS19235	962319..962606	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FDZ90_RS19240	962734..963189	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
FDZ90_RS19245	963511..963663	+	153	WP_001884520.1	DUF645 family protein	-
FDZ90_RS19250	963865..964362	-	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
FDZ90_RS19255	964359..964631	-	273	WP_000246253.1	DUF1778 domain-containing protein	Antitoxin
FDZ90_RS19260	964862..965530	+	669	WP_000043871.1	hypothetical protein	-
FDZ90_RS19265	965682..965969	+	288	WP_000426470.1	hypothetical protein	-
FDZ90_RS19270	966110..966481	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
FDZ90_RS19275	966548..966973	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
FDZ90_RS19280	966970..967503	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
FDZ90_RS19285	967705..967962	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FDZ90_RS19290	967950..968252	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FDZ90_RS19295	968486..969403	+	918	WP_000186333.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	882589..977840	95251
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	catB9	-	887669..977464	89795
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0.009	flank	IS/Tn	-	-	958940..959659	719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T125047 WP_000982260.1 NZ_CP040173:c964362-963865 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T125047 NZ_CP040173:c964362-963865 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9683.22 Da
• Isoelectric Point: 6.2988

>AT125047 WP_000246253.1 NZ_CP040173:c964631-964359 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT125047 NZ_CP040173:c964631-964359 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1

Antitoxin

• AlphaFold DB: A0A0F2IC79