Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 326876..327642 Replicon chromosome
Accession NZ_CP047304
Organism Vibrio cholerae O1 biovar El Tor strain E7946

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag GTF74_RS15200 Protein ID WP_000982260.1
Coordinates 326876..327373 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag GTF74_RS15205 Protein ID WP_000246253.1
Coordinates 327370..327642 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTF74_RS15150 322318..323022 + 705 WP_000087610.1 HNH endonuclease -
GTF74_RS15155 323180..323518 + 339 WP_000713853.1 hypothetical protein -
GTF74_RS15160 323672..323989 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTF74_RS15165 324008..324250 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTF74_RS15170 324494..324880 + 387 WP_001015867.1 NUDIX hydrolase -
GTF74_RS15175 324937..325050 + 114 WP_001900214.1 hypothetical protein -
GTF74_RS15180 324999..325280 + 282 WP_001894429.1 DUF1289 domain-containing protein -
GTF74_RS15185 325256..325414 - 159 WP_001886450.1 DUF1196 family protein -
GTF74_RS15190 325538..326074 + 537 WP_000469482.1 GNAT family N-acetyltransferase -
GTF74_RS15195 326211..326726 + 516 WP_001201509.1 lipocalin family protein -
GTF74_RS15200 326876..327373 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
GTF74_RS15205 327370..327642 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
GTF74_RS15210 328035..328160 + 126 WP_001900195.1 DUF645 family protein -
GTF74_RS15215 328176..328214 + 39 WP_106019118.1 hypothetical protein -
GTF74_RS15220 328410..328856 + 447 WP_000006157.1 hypothetical protein -
GTF74_RS15225 329004..329273 - 270 WP_000277238.1 type II toxin-antitoxin system YafQ family toxin -
GTF74_RS15230 329266..329544 - 279 WP_001258569.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
GTF74_RS15235 329830..330108 + 279 WP_001921601.1 DUF3709 domain-containing protein -
GTF74_RS15240 330360..330566 + 207 Protein_294 DUF3709 domain-containing protein -
GTF74_RS15245 330766..330867 + 102 WP_001921603.1 hypothetical protein -
GTF74_RS15250 330857..331243 + 387 WP_000703163.1 VOC family protein -
GTF74_RS15255 331438..331689 + 252 WP_001890111.1 TIGR03643 family protein -
GTF74_RS15260 331662..331811 + 150 WP_071908333.1 DUF3265 domain-containing protein -
GTF74_RS15265 331903..332145 + 243 WP_000107461.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 307032..436751 129719
inside Integron - - 312112..436375 124263


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T145884 WP_000982260.1 NZ_CP047304:c327373-326876 [Vibrio cholerae O1 biovar El Tor]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T145884 NZ_CP062705:c4713289-4712975 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGTAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT145884 WP_000246253.1 NZ_CP047304:c327642-327370 [Vibrio cholerae O1 biovar El Tor]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT145884 NZ_CP062705:c4713525-4713292 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
PDB 1Y9B
AlphaFold DB A0A0F2IC79

References