58398

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 395548..396046 Replicon chromosome
Accession NZ_CP013318
Organism Vibrio cholerae strain NCTC5395

Toxin (Protein)


Gene name - Uniprot ID Q9KMK7
Locus tag ASZ85_RS16205 Protein ID WP_000589156.1
Coordinates 395548..395814 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9KMK6
Locus tag ASZ85_RS16210 Protein ID WP_000643598.1
Coordinates 395801..396046 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ85_RS21045 390688..390777 + 90 WP_131065139.1 acetyltransferase -
ASZ85_RS16165 390770..391633 + 864 WP_001900189.1 hypothetical protein -
ASZ85_RS16175 391876..392154 + 279 WP_000280319.1 DUF3709 domain-containing protein -
ASZ85_RS16180 392423..392959 + 537 WP_000644491.1 nucleotidyltransferase family protein -
ASZ85_RS21450 393078..393239 + 162 WP_000481782.1 hypothetical protein -
ASZ85_RS16185 393428..393538 + 111 WP_071908335.1 DUF3265 domain-containing protein -
ASZ85_RS16190 393590..393943 + 354 WP_000384364.1 hypothetical protein -
ASZ85_RS16195 394106..394573 + 468 WP_001289288.1 OsmC family protein -
ASZ85_RS16200 394701..395363 + 663 WP_000960654.1 hypothetical protein -
ASZ85_RS16205 395548..395814 + 267 WP_000589156.1 BrnT family toxin Toxin
ASZ85_RS16210 395801..396046 + 246 WP_000643598.1 hypothetical protein Antitoxin
ASZ85_RS16215 396142..396936 + 795 WP_001911581.1 hypothetical protein -
ASZ85_RS21455 397039..397167 + 129 WP_071908338.1 general secretion pathway protein GspI -
ASZ85_RS16230 397228..397410 + 183 WP_000923182.1 DUF645 family protein -
ASZ85_RS16235 397626..398630 + 1005 WP_000964920.1 LD-carboxypeptidase -
ASZ85_RS16240 398783..399142 + 360 WP_001071541.1 VOC family protein -
ASZ85_RS16245 399415..399648 + 234 WP_001890112.1 DUF3709 domain-containing protein -
ASZ85_RS16250 399916..400422 + 507 WP_000393074.1 hypothetical protein -
ASZ85_RS16255 400579..400965 + 387 WP_000703163.1 VOC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 338285..496833 158548
inside Integron - - 343365..496457 153092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(6-81)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10092.34 Da        Isoelectric Point: 7.3171

>T58398 WP_000589156.1 NZ_CP013318:395548-395814 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Download         Length: 267 bp

>T58398 NZ_CP013318:395548-395814 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9478.77 Da        Isoelectric Point: 6.4832

>AT58398 WP_000643598.1 NZ_CP013318:395801-396046 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Download         Length: 246 bp

>AT58398 NZ_CP013318:395801-396046 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A271VMP7

Antitoxin

Source ID Structure
AlphaFold DB A0A271VME2

References